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Related Experiment Video

Updated: Mar 31, 2026

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Imaging Septum Formation by Fluorescence Microscopy.

Juan Carlos Ribas1, Juan Carlos G Cortés2

  • 1Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC) / Universidad de Salamanca, C/ Zacarias Gonzalez 1, 37007, Salamanca, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|November 1, 2015
PubMed
Summary

This study details methods for visualizing fungal cell division septa using fluorescence microscopy. Researchers developed new techniques to observe septum formation and progression in Schizosaccharomyces pombe, aiding the study of cytokinesis.

Keywords:
Calcofluor whiteCell wallCytokinesisGlucan synthasePolysaccharideSeptumβ(1,3)-glucan

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Area of Science:

  • Cell Biology
  • Mycology
  • Microscopy

Background:

  • Fungal cytokinesis involves actomyosin ring contraction and septum formation.
  • The fission yeast Schizosaccharomyces pombe forms a unique β(1,3)glucan-rich primary septum.
  • Septum synthesis is crucial for proper ring contraction during cell division.

Purpose of the Study:

  • To develop and describe methods for imaging fungal septum structure.
  • To analyze septum progression during cytokinesis using time-lapse microscopy.
  • To investigate the molecular links between the septum and cytokinetic machinery.

Main Methods:

  • Transmission electron microscopy (TEM) for detailed septum structure analysis.
  • Fluorescence microscopy for visualizing septum formation and progression.
  • Time-lapse microscopy to observe dynamic changes during cytokinesis.

Main Results:

  • The study describes established methods for fluorescence imaging of fungal septa.
  • Time-lapse microscopy allows for detailed analysis of septum progression.
  • These methods facilitate the study of molecular mechanisms connecting septum synthesis to cytokinesis.

Conclusions:

  • New fluorescence microscopy methods are presented for studying fungal cell division.
  • The techniques enable detailed observation of septum formation and its role in cytokinesis.
  • Further research can utilize these methods to explore the molecular basis of fungal cytokinesis.