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Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

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Published on: March 1, 2013

Layer-by-layer inorganic/polymeric nanoparticles for kinetically controlled multigene delivery.

Corey J Bishop¹, Allen L Liu¹, David S Lee¹

¹Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Translational Tissue Engineering Center, Baltimore, Maryland, 21231.

Journal of Biomedical Materials Research. Part A

|November 1, 2015

Summary

This summary is machine-generated.

Researchers developed a novel nanoparticle for nonviral gene delivery, capable of carrying multiple genes with distinct expression timelines. This advancement offers enhanced control over cellular processes for gene therapy applications.

Keywords:

cell engineering gene delivery gold nanoparticle layer-by-layer poly(beta-amino ester)

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Published on: February 25, 2021

Area of Science:

Biomaterials Science
Gene Therapy
Nanotechnology

Background:

Nonviral gene delivery offers a safer alternative to viral methods for treating diseases.
Current gene therapy often requires delivering multiple genes with controlled expression timing for optimal efficacy.
Polymeric nanoparticles are versatile for gene delivery, capable of carrying large genes and easier to manufacture than viral vectors.

Purpose of the Study:

To develop a layer-by-layer (LbL) nanoparticle capable of theranostic applications.
To incorporate two distinct plasmid types with differing expression profiles into a single nanoparticle.
To enable temporal control over exogenous DNA expression for improved cellular control.

Main Methods:

Fabrication of a layer-by-layer (LbL) nanoparticle system.
Incorporation of two different plasmid DNA types into the nanoparticle structure.
Evaluation of the nanoparticle's capacity for delivering multiple genes with varied expression kinetics.

Main Results:

Successful development of an LbL nanoparticle for gene delivery.
Demonstrated ability to encapsulate two plasmid types with distinct expression profiles.
Established a platform for temporally controlled gene expression from delivered DNA.

Conclusions:

The developed LbL nanoparticle is a promising platform for advanced gene therapy.
Temporal control over gene expression can be achieved using this nanoparticle system.
This approach offers enhanced control over cellular microenvironments, differentiation, and cell fate.