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Chitin from Penaeus merguiensis via microbial fermentation processing and antioxidant activity
Fatemeh Sedaghat1, Morteza Yousefzadi1, Hojjat Toiserkani2
1Department of Marine Biology, Faculty of Marine Science and Technology, Hormozgan University, Bandar Abbas, Iran.
International Journal of Biological Macromolecules
|November 1, 2015
Summary
Pseudomonas aeruginosa effectively demineralized and deproteinized shrimp waste for chitin extraction. This bacterium shows significant potential for processing marine crustacean waste, yielding valuable chitin and chitosan derivatives.
Area of Science:
- Biotechnology
- Marine Biology
- Microbiology
Background:
- Shrimp waste is a rich source of chitin, a valuable biopolymer.
- Efficient extraction of chitin requires effective demineralization and deproteinization of the waste.
- Protease-producing bacteria offer a sustainable approach for waste bioprocessing.
Purpose of the Study:
- To investigate the efficiency of three protease-producing bacteria (Pseudomonas aeruginosa, Serratia marcescens, Bacillus pumilus) in demineralizing and deproteinizing shrimp waste for chitin extraction.
- To evaluate the antioxidant properties of chitin and its derivative, chitosan, obtained from the process.
- To assess the antioxidant potential of bacterial hydrolysates.
Main Methods:
- Screening of P. aeruginosa, S. marcescens, and B. pumilus for demineralization and deproteinization of shrimp waste.
- Chitin extraction followed by deacetylation using NaOH to produce chitosan.
- Assessing antioxidant activity of chitosan using spectrophotometric methods (DO 700 nm, DO 695 nm).
- Evaluating antioxidant potential of bacterial hydrolysates (reducing power, total antioxidant capacity).
Main Results:
- P. aeruginosa demonstrated the highest deproteinization (74.76%) and demineralization (78.46%) efficiencies.
- S. marcescens showed the lowest demineralization and deproteinization rates.
- Chitosan exhibited significant antioxidant activity at a concentration of 1,000 μg/ml.
- S. marcescens and B. pumilus hydrolysates showed high reducing power and total antioxidant capacity, respectively.
Conclusions:
- P. aeruginosa is highly effective in removing proteins and minerals from shrimp waste compared to S. marcescens and B. pumilus.
- The use of P. aeruginosa is recommended for efficient protein and mineral removal in marine crustacean waste processing.
- The study highlights the potential of bacterial bioprocessing for chitin extraction and the antioxidant properties of derived chitosan.
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