Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Maxam-Gilbert Sequencing

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Structure-selective binding and bioactivity of chlorine-substituted cyanine dyes with DNA, RNA, and lipid membranes.

Bioorganic chemistry·2026

Same author

Uracil-Peptide-Pyrene Conjugates as Cu<sup>2+</sup>-Sensitive Probes Targeting DNA, RNA, and Biorelevant Model Membranes.

Chembiochem : a European journal of chemical biology·2026

Same author

The biophysical characterization of the transcription factor MntR from Mycobacterium tuberculosis and its selected mutants.

International journal of biological macromolecules·2026

Same author

Synthesis and Characterization of Thermostable Antimicrobial Peptide-DNAzyme Conjugates for the Proof-of-Concept Detection of <i>E. coli</i>.

ACS omega·2026

Same author

Molecular Pathways Affected by Sulfonylpurine Derivatives in 2D and 3D HeLa Cell Models.

Molecules (Basel, Switzerland)·2025

Same author

Synthesis, Biological Evaluation, and Interaction with Calf Thymus-DNA of Mono- and Diamidino-Substituted Imidazo[4,5-b]pyridines.

ChemMedChem·2025

Search research articles

Related Experiment Video

Updated: Mar 30, 2026

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Advances in Peptide-based DNA/RNA-Intercalators.

Josipa Matić, Lidija-Marija Tumir, Marijana Radić Stojković

¹Laboratory for Study of Interactions of Biomacromolecules, Division of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Zagreb, Croatia. pianta@irb.hr.

Current Protein & Peptide Science

|November 3, 2015

Summary

This summary is machine-generated.

Nature utilizes peptide-nucleic acid interactions for vital processes. Synthetic peptides offer versatile platforms for creating novel DNA/RNA binders and intercalators with unique properties.

More Related Videos

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Published on: April 3, 2014

Related Experiment Videos

Last Updated: Mar 30, 2026

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Published on: April 3, 2014

Area of Science:

Biochemistry
Molecular Biology
Medicinal Chemistry

Background:

Peptide-nucleic acid (DNA/RNA) interactions are fundamental to biological processes.
Naturally occurring molecules like echinomycin demonstrate synergistic activity through combined peptide and DNA intercalator components.
Synthetic peptides serve as recognition units or carriers for bioactive substituents, exemplified by peptide nucleic acids (PNAs).

Purpose of the Study:

To explore the synthetic versatility of peptide backbones for creating novel DNA/RNA interacting agents.
To investigate the potential of peptide-based structures as carriers for bioactive substituents.
To develop new intercalators and poly-intercalators with unique DNA/RNA binding properties.

Main Methods:

Utilizing versatile synthetic approaches for short peptides.
Incorporating peptide backbones as carriers for bioactive substituents.
Synthesizing novel intercalators and poly-intercalators based on peptide structures.

Main Results:

Demonstrated the utility of synthetic peptides as recognition units for DNA/RNA grooves.
Showcased peptide backbones as effective carriers for diverse bioactive moieties.
Synthesized novel peptide-based intercalators and poly-intercalators exhibiting intriguing DNA/RNA interaction profiles.

Conclusions:

Synthetic peptides provide a flexible scaffold for designing novel nucleic acid-binding molecules.
Peptide-based conjugates offer a promising avenue for developing agents with tailored DNA/RNA interacting properties.
Further research into peptide-nucleic acid interactions can yield innovative therapeutic and diagnostic tools.