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Effect of Tongxinluo on nerve regeneration in mice with diabetic peripheral neuropathy
1Henan Provincial People's Hospital Department of Neurology Zhengzhou China lixuehn@yeah.net.
Abstract:
Diabetic peripheral neuropathy (DPN) is a common complication of diabetes. This study aims to investigate the effects of Tongxinluo on the nerve regeneration in diabetic peripheral neuropathy mice. Forty Specefic Pathogen Free (SPF) male KK/Upj—Ay mice were divided into diabetes group, diabetes with high dose Tongxinluo (4g/kg) (D+H), diabetes with mid dose Tongxinluo (2g/kg) (D+M), and diabetes with low dose Tongxinluo (1g/kg) (D+L) groups. Fasting blood glucose (FPG), heat pain threshold, motor nerve conduction velocity (MNCV), insulin—like growth factor—1 (IGF1), activator protein 1 (c—fos), nerve growth factor (NGF), and basic fibroblast growth factor (BFGF) were measured. Results indicated that FPG of diabetes group was significantly higher than that of control group. Heat pain threshold and MNCV were significantly lowered in diabetes group. Expression levels of IGF1, NGF and BFGF were significantly lower than that of control, whereas c—fos expression was significantly higher than that of control group. Tongxinluo treatment (D+M and D+H) significantly up—regulated heat pain threshold, MNCV, and IGF1, NGF and BFGF expression, but decreased c—fos expresson when compared to that of diabetes group. In conclusion, Tongxinluo can ameliorate diabetic peripheral neuropathy, improve MNCV, and promote nerve regeneration. The underlying mechanism needs to be further elucidated.
Insights
Tongxinluo treatment improved nerve regeneration in diabetic peripheral neuropathy (DPN) mice. It enhanced nerve function and growth factors, suggesting a therapeutic potential for DPN.
Area of Science:
- Neuroscience
- Pharmacology
- Diabetology
Background:
- Diabetic peripheral neuropathy (DPN) is a prevalent complication of diabetes mellitus.
- DPN significantly impairs nerve function and quality of life.
Purpose of the Study:
- To investigate the therapeutic effects of Tongxinluo on nerve regeneration in a mouse model of DPN.
- To evaluate the impact of Tongxinluo on nerve function, growth factors, and related signaling pathways.
Main Methods:
- Forty male KK/Upj-Ay mice were allocated into control, diabetes, and three Tongxinluo-treated groups (low, mid, high dose).
- Measurements included fasting blood glucose, thermal hyperalgesia, motor nerve conduction velocity (MNCV), and protein expression of IGF1, c-fos, NGF, and BFGF.
Main Results:
- Diabetes significantly increased blood glucose and c-fos expression while decreasing thermal pain threshold, MNCV, and IGF1, NGF, and BFGF levels.
- Tongxinluo treatment, particularly at mid and high doses, significantly improved thermal pain threshold, MNCV, and normalized the expression of growth factors and c-fos.
Conclusions:
- Tongxinluo demonstrates a protective and regenerative effect on peripheral nerves in diabetic mice.
- The findings suggest Tongxinluo as a potential therapeutic agent for ameliorating DPN by promoting nerve regeneration and function.
