Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mycobacteriophage vector systems.

W R Jacobs1, S B Snapper, M Tuckman

  • 1Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461.

Reviews of Infectious Diseases
|March 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spontaneous food allergy in Was<sup>-/-</sup> mice occurs independent of FcεRI-mediated mast cell activation.

Allergy·2017
Same author

Monoclonal antibodies against mycobacterial antigens.

Immunology today·2014
Same author

Mycobacterium tuberculosis Lsr2 is a global transcriptional regulator required for adaptation to changing oxygen levels and virulence.

mBio·2014
Same author

Elevated levels of leukotriene C4 synthase mRNA distinguish a subpopulation of eosinophilic oesophagitis patients.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2013
Same author

Transposon mutagenesis in mycobacteria using conditionally replicating mycobacteriophages.

Methods in molecular medicine·2011
Same author

The multifunctional histone-like protein Lsr2 protects mycobacteria against reactive oxygen intermediates.

Proceedings of the National Academy of Sciences of the United States of America·2009
Same journal

Fatal necrotizing otitis externa in a patient with AIDS.

Reviews of infectious diseases·1991
Same journal

Use of the polymerase chain reaction for the specific and direct detection of Clostridium difficile in human feces.

Reviews of infectious diseases·1991
Same journal

A new case of meningitis due to Pasteurella multocida.

Reviews of infectious diseases·1991
Same journal

Disseminated pelvic actinomycosis presenting as metastatic carcinoma: association with the progestasert intrauterine device.

Reviews of infectious diseases·1991
Same journal

Genetically engineered attenuated herpes simplex viruses.

Reviews of infectious diseases·1991
Same journal

Role of altered drug metabolism in virus-drug interactions.

Reviews of infectious diseases·1991
See all related articles

Researchers developed novel shuttle phasmids for genetic manipulation of mycobacteria. These versatile vectors enable DNA introduction and stable integration, advancing molecular studies in diverse mycobacterial species.

Area of Science:

  • Microbiology
  • Molecular Genetics
  • Recombinant DNA Technology

Background:

  • Molecular genetic studies of mycobacteria require efficient DNA introduction methods.
  • Existing methods for DNA delivery into mycobacteria have limitations.
  • Development of novel vectors is crucial for advancing mycobacterial genetics.

Purpose of the Study:

  • To construct and characterize novel Escherichia coli-Mycobacterium shuttle vectors.
  • To enable efficient cloning and expression of foreign DNA in mycobacteria.
  • To facilitate the development of molecular genetic systems for diverse mycobacterial species.

Main Methods:

  • Construction of shuttle phasmids by inserting E. coli cosmids into mycobacteriophage DNA.
  • Utilizing bacteriophage lambda cos sequences for in vitro packaging and cloning.

Related Experiment Videos

  • Testing transduction capabilities across various mycobacterial species.

    • Main Results:

    • Shuttle phasmids replicate as plasmids in E. coli and as phages in mycobacteria.
    • Vectors support stable integration and expression of cloned DNA via lysogenization.
    • Successful transduction demonstrated across a wide range of mycobacterial species.

    Conclusions:

    • Shuttle phasmids represent a significant advancement for mycobacterial molecular genetics.
    • These vectors offer a versatile platform for gene cloning, expression, and manipulation.
    • The developed system holds promise for creating robust genetic tools for mycobacteria.