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Updated: Mar 30, 2026
Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
Published on: September 6, 2017
[Identification of A Novel HLA Allele DRB1 * 16:36 by Sequencing-Based Typing]
Li-Jun Lu1,2, Wei-Ze Zuo2, Wan-Jiang Zhang1
1State Key Laboratory, Ministry of Education, Shihezi University Medical College; Shihezi 832000, Xinjiang Uygur Autonomous Region, China.
Objective:
To identify a novel HLA allele DRB1 * 16:36 from a Uygur woman.
Methods:
PCR-SBT technology was applied to the extracted DNA for genotyping, and a possible new gene was sequenced by using sequence specific primers and single stranded SBT. This novel allele was compared with known most homologous gene sequences and their difference was analyzed.
Results:
This novel allele was different from HLA alle DRB1 * 16:23, and had highest similarity in 2 nucleotides at position 227 A→T and 236 T→C in exon 2, resulting in 3 amino acid changes from Tyr to Phe at codon 47 and Val to Ala at codon 50. The sequence of this novel allele had been submitted to GenBank.
Conclusion:
This HLA allele DRB1 * 16:23 has been confirmed to be a novel allele, and has been officially named DRB1 * 16:36 by the World Health Organization (WHO) Nomenclature Committee in May 2015.
