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Updated: Mar 30, 2026

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
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In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

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Structural basis for catalytic activation by the human ZNF451 SUMO E3 ligase

Laurent Cappadocia1, Andrea Pichler2, Christopher D Lima1,3

  • 1Structural Biology Program, Sloan Kettering Institute, New York, New York, USA.

Nature Structural & Molecular Biology
|November 3, 2015
PubMed
Summary

The ZNF451 protein acts as a SUMO E3 ligase, facilitating SUMOylation by interacting with E2 enzymes and SUMO proteins. This study defines its catalytic mechanism and SUMO2 specificity.

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