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Related Experiment Video

Updated: Mar 30, 2026

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Published on: May 28, 2021

SIMcheck: a Toolbox for Successful Super-resolution Structured Illumination Microscopy.

Graeme Ball¹, Justin Demmerle¹, Rainer Kaufmann^1,2

¹Department of Biochemistry, University of Oxford, Oxford, UK.

Scientific Reports

|November 4, 2015

Summary

This summary is machine-generated.

SIMcheck is a new ImageJ plugin suite that helps users generate high-quality super-resolution microscopy data. It identifies common issues in 3D-SIM imaging and improves data analysis for cell biology research.

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Area of Science:

Microscopy
Cell Biology
Image Analysis

Background:

Three-dimensional structured illumination microscopy (3D-SIM) offers super-resolution fluorescence imaging capabilities.
Acquiring high-quality 3D-SIM data can be challenging, especially for users without specialized expertise.

Purpose of the Study:

To introduce SIMcheck, a novel ImageJ plugin suite designed to address common challenges in 3D-SIM data acquisition and processing.
To provide objective tools for assessing 3D-SIM data quality and resolution.

Main Methods:

Development of a suite of ImageJ plugins named SIMcheck.
Implementation of objective control parameters for data quality assessment.
Inclusion of advanced calibration tools and image processing utilities.

Main Results:

SIMcheck enables users to identify and mitigate common artifacts in 3D-SIM datasets.
The software provides objective metrics for evaluating image resolution and data quality.
It offers versatile tools applicable to various commercial and custom 3D-SIM platforms.

Conclusions:

SIMcheck empowers researchers, including non-specialists, to achieve reliable super-resolution imaging using 3D-SIM.
This open-source software facilitates the routine application of 3D-SIM in cell biology.
It promotes consistent and high-quality data generation across different 3D-SIM systems.