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[Low-molecular-weight DNA in blood plasma and cerebrospinal fluid of patients with acute stroke]
I N Vasilyeva1, I A Voznjouk2, V G Bespalov3
1Petrov Research Institute of Oncology, St. Petersburg.
Aim:
To characterize the value of low-molecular-weight DNA in blood plasma and cerebrospinal fluid for the diagnosis of acute stroke of ischemic and hemorrhagic types early after the onset of the disease.
Material And Methods:
DNA from plasma and cerebrospinal fluid was isolated by phenol deproteinization, analyzed by gradient polyacrylamide electrophoresis. Content of low-molecular-weight DNA was determined on the electrophoregram by comparison with standards.
Results:
Authors discovered the differences in the changes in the content of low-molecular-weight DNA in the blood plasma of patients with acute ischemic and hemorrhagic types of cerebrovascular accident within 3 days after onset. Patients with hemorrhagic stroke were characterized by an increase in low-molecular-weight DNA after 3 hours, while patients with ischemic stroke after 24 hours from onset. Low-molecular-weight DNA was also detected in the cerebrospinal fluid of patients with ischemia during the first day of the disease.
Conclusion:
The results indicate a role of apoptosis in the formation of ischemic lesion.
