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Updated: Mar 30, 2026
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Plasma miR-1, miR-208, miR-499 as potential predictive biomarkers for acute myocardial infarction: An independent
Xian Liu1, Zhanwei Fan2, Tianshu Zhao3
1Department of Cardiology, The Fourth Clinical Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China.
Background:
It has been demonstrated that circulating muscle-enriched miR-1 and the cardiac-specific miR-208 and miR-499 might leak out of the necrotic myocardium and release into the circulation during the early stages of acute myocardial infarction (AMI). This study aims to investigate potential predictive value of the three miRNAs in AMI.
Methods:
Plasma samples were taken from 70 AMI patients and 72 healthy controls. The expression levels of target miRNAs were measured by qRT-PCR. Student's t test and χ(2) test were applied to test the significance of the differences between AMI patients and controls. Additionally, a meta-analysis about the prediction accuracy of the three miRNAs in AMI was performed to summarize all the results from available studies.
Results:
The expression of plasma miR-1, miR-208 and miR-499 were all significantly elevated in AMI patients compared with controls. By receiver operating characteristic (ROC) curve analysis, plasma miR-1, miR-208 and miR-499 were demonstrated to be potential biomarkers for the prediction of AMI with AUC values of 0.81, 0.72, and 0.88, respectively. The meta-analysis consisted of nine studies for miR-1, eight studies for miR-208 and eight studies for miR-499. The summary estimates of miR-1, miR-208 and miR-499 were 0.73, 0.80 and 0.83 for sensitivity, 0.82, 0.95 and 0.90 for specificity, 0.84, 0.89 and 0.91 for AUC, respectively.
Conclusion:
In summary, we confirmed the predictive values of plasma miR-1, miR-208 and miR-499 in AMI. In contrast to miR-1, the cardiac-specific miR-208 and miR-499 were supposed to be more reliable as biomarkers in AMI screening and prediction.
Insights
Circulating microRNAs (miRNAs) miR-1, miR-208, and miR-499 are elevated in acute myocardial infarction (AMI) patients. Cardiac-specific miR-208 and miR-499 show greater reliability as biomarkers for AMI prediction.
Area of Science:
- Biochemistry
- Molecular Biology
- Cardiology
Background:
- Circulating muscle-enriched miR-1 and cardiac-specific miR-208/miR-499 may be released from necrotic myocardium during acute myocardial infarction (AMI).
- Investigating the predictive value of these miRNAs in AMI is crucial for early diagnosis and management.
Purpose of the Study:
- To evaluate the predictive potential of plasma miR-1, miR-208, and miR-499 as biomarkers for acute myocardial infarction (AMI).
Main Methods:
- Plasma samples were collected from 70 AMI patients and 72 healthy controls.
- MicroRNA expression levels were quantified using qRT-PCR.
- Statistical analyses included Student's t test, χ(2) test, ROC curve analysis, and a meta-analysis of existing studies.
Main Results:
- Plasma levels of miR-1, miR-208, and miR-499 were significantly higher in AMI patients compared to controls.
- ROC analysis indicated potential biomarker utility with AUC values of 0.81 (miR-1), 0.72 (miR-208), and 0.88 (miR-499).
- Meta-analysis revealed pooled sensitivity, specificity, and AUC values for miR-1, miR-208, and miR-499, confirming their diagnostic accuracy.
Conclusions:
- Plasma miR-1, miR-208, and miR-499 demonstrate significant predictive value in acute myocardial infarction (AMI).
- Cardiac-specific miR-208 and miR-499 are suggested to be more reliable biomarkers for AMI screening and prediction compared to miR-1.
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