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Updated: Mar 30, 2026

Author Spotlight: Accelerating Diagnostic Accuracy with Direct Identification of Gram-Negatives from Blood Culture Bottles
Published on: May 24, 2024
Performance of Vitek MS in identifying nontuberculous mycobacteria from MGIT liquid medium and Lowenstein-Jensen
Jan Kehrmann1, Ann-Kathrin Schoerding1, Roshni Murali1
1Institute of Medical Microbiology, University Hospital Essen, University Duisburg-Essen, Essen, Germany.
Abstract:
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is a fast and inexpensive method for bacterial identification. The aim of this study was to analyze the performance of Vitek MS in identifying 160 nontuberculous mycobacterial isolates of 24 species from Lowenstein-Jensen solid medium and BACTEC MGIT 960 liquid medium using a bead-based method. The system correctly identified 76.9% of the isolates (123 of 160) cultivated on solid medium and 76.9% (123 of 160) of positive liquid cultures. None of the isolates included in the study was misidentified. Although the overall performance of Vitek MS with the SARAMIS 4.12 database was comparable in identifying mycobacterial species grown on solid medium and in liquid medium, the identification rate varied notably between the various species analyzed, which currently limits the utility for identification in routine diagnostics for some species.
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