Related Experiment Video
Updated: Mar 30, 2026
Formation of Human Prostate Epithelium Using Tissue Recombination of Rodent Urogenital Sinus Mesenchyme and Human Stem Cells
Published on: June 22, 2013
Preparation of Urogenital Sinus Mesenchymal Cells for Prostate Tissue Recombination Models
Yang Zong1, Andrew S Goldstein2, Owen N Witte3
1Howard Hughes Medical Institute, University of California, Los Angeles, California 90095;
Abstract:
An appropriate microenvironment provided by the mesenchyme is important for establishing tissue recombination models for epithelial cancer. Urogenital sinus mesenchymal (UGSM) cells derived from embryonic rodent show potent inductive effects for prostate regeneration. Genetic manipulation of these mesenchymal cells allows us to define the contribution of the tumor microenvironment to prostate cancer development. This protocol describes preparation and propagation of murine UGSM cells in culture.
