Dissociated Prostate Regeneration under the Renal Capsule
Yang Zong1, Andrew S Goldstein2, Owen N Witte3
1Howard Hughes Medical Institute, University of California, Los Angeles, California 90095;
Cold Spring Harbor Protocols
|November 4, 2015
Abstract:
Tissue recombination models are useful for studying cancer initiation, progression, and metastasis. They also provide an in vivo environment in which to investigate the functional role of stem cells in tissue repair. In this protocol, we describe in detail the dissociated prostate regeneration assay. Dissociated adult murine prostate cells are combined with embryonic urogenital sinus mesenchymal cells and implanted under the renal capsule. Morphological tissue structures with appropriate epithelial-stroma interactions are reconstituted in the grafts.
