Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbiota of the Large Intestine01:27

Microbiota of the Large Intestine

40
The large intestine hosts the most densely populated microbial ecosystem in the human body. This complex community primarily consists of anaerobic bacteria, with Bacillota (formerly Firmicutes) and Bacteroidota (formerly Bacteroidetes) as the predominant groups. The distribution of these microbes varies along different sections of the large intestine, influenced by local environmental factors such as oxygen availability and nutrient composition.The cecum, located at the beginning of the large...
40
Microbes in Food Production01:29

Microbes in Food Production

227
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
227
Development of Human Microbiota01:30

Development of Human Microbiota

34
The human microbiota begins developing at birth and undergoes continual change as we age. Infancy marks a critical period of microbial sensitivity, offering a “window of opportunity” during which beneficial microbes help mature the immune system. By age three, children typically develop a more stable and diverse microbial community. Newborns acquire microbes from their immediate environment; vaginal delivery favors maternal vaginal microbes, while cesarean births favor microbes from...
34
Microbes in the Production of Fermented Foods01:27

Microbes in the Production of Fermented Foods

182
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
182
Evolution of New Traits in Microbes01:24

Evolution of New Traits in Microbes

104
Microorganisms evolve rapidly due to their large population sizes and short generation times, often exhibiting measurable changes within days under laboratory conditions. Natural selection acts on standing genetic variation, enabling the retention and amplification of beneficial traits that confer fitness advantages in changing environments.Adaptive Pigment Regulation in RhodobacterIn Rhodobacter, a genus of purple non-sulfur bacteria, light-harvesting pigments such as bacteriochlorophyll and...
104
Functions of the Gut Microbiota01:18

Functions of the Gut Microbiota

46
The gut microbiota includes trillions of microorganisms that colonize the human gastrointestinal tract, including bacteria, archaea, viruses, and fungi. This complex ecosystem plays a critical role in maintaining intestinal and systemic health. Most of these microbes inhabit the large intestine, establishing a relatively stable and diverse community that contributes to gut homeostasis through various metabolic, immunological, and protective mechanisms.Dominant bacterial phyla, such as...
46

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ruminosignatures associated with methane emissions and feed efficiency across geographies and cattle breeds.

The ISME journal·2026
Same author

Cannabis consumption is associated with altered steroid metabolism in young men.

Communications medicine·2026
Same author

Heterologous internal calibration for multiplexed internal quantification in targeted LC-MS/MS bioanalysis.

Journal of pharmaceutical and biomedical analysis·2026
Same author

Harnessing gastrointestinal microbial co-association networks to predict feed efficiency and methane emissions across beef and dairy cattle.

Microbial genomics·2026
Same author

Characterization of residual kidney function in chronic hemodialysis patients using plasma metabolomics.

Scientific reports·2026
Same author

Lipidomic profiling of extracellular vesicles from breast and metastatic triple-negative breast cancer cell lines for identification of potential biomarkers.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2026

Related Experiment Video

Updated: Mar 30, 2026

Metagenomic Analysis of Silage
08:43

Metagenomic Analysis of Silage

Published on: January 13, 2017

19.3K

Rumen microbial communities influence metabolic phenotypes in lambs.

Diego P Morgavi1, Estelle Rathahao-Paris2, Milka Popova1

  • 1Institute National de la Recherche Agronomique, UMR1213 Herbivores, Clermont Université, VetAgro Sup, UMR Herbivores Clermont-Ferrand, France.

Frontiers in Microbiology
|November 4, 2015
PubMed
Summary

Rumen microbes significantly impact ruminant health and nutrition. This study reveals how protozoa and microbial diversity influence lamb digestive function and metabolic profiles, identifying potential biomarkers.

Keywords:
gut microbial colonizationhost-microbe interactionrumen archaearumen bacteriarumen protozoaurine metabolome

More Related Videos

Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings
14:40

Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings

Published on: October 25, 2015

10.0K
Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments
10:31

Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments

Published on: July 24, 2018

57.4K

Related Experiment Videos

Last Updated: Mar 30, 2026

Metagenomic Analysis of Silage
08:43

Metagenomic Analysis of Silage

Published on: January 13, 2017

19.3K
Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings
14:40

Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings

Published on: October 25, 2015

10.0K
Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments
10:31

Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments

Published on: July 24, 2018

57.4K

Area of Science:

  • Rumen microbiology
  • Animal nutrition
  • Metabolomics

Background:

  • The rumen microbiota is crucial for ruminant health and nutrition, but its complex interactions with the host are not fully understood.
  • Identifying specific microbial groups and their impact on host functions is essential for optimizing animal health and productivity.

Purpose of the Study:

  • To investigate host-microbe associations by simultaneously analyzing rumen microbiota and metabolic phenotypes in lambs.
  • To identify potential biomarkers for digestive functions influenced by microbial composition.

Main Methods:

  • Twin lambs were subjected to distinct microbial acquisition protocols (isolation vs. gavage with protozoa-containing or depleted rumen fluid).
  • Rumen microbiota, fermentation parameters, digestibility, and growth were monitored up to 31 weeks.
  • Urinary metabolomics was employed to correlate microbial structure with metabolic phenotypes.

Main Results:

  • Isolated microbiota lacked protozoa, showing lower diversity but unaffected digestibility.
  • Exposure to adult microbiota increased bacterial and archaeal diversity; Methanomassiliicoccales displaced Methanosphaera.
  • Protozoa significantly altered functional traits and bacterial structure (e.g., Ruminococcaceae, Lachnospiraceae, Prevotellaceae, Clostridiaceae, Veillonellaceae).
  • Urinary metabolome analysis revealed discriminant metabolites linked to amino acid and protein pathways, with a negative interaction between Methanomassiliicoccales and trimethylamine N-oxide.

Conclusions:

  • Rumen microbial composition, particularly the presence of protozoa, profoundly influences host phenotype, including digestive function and metabolism.
  • Metabolomics serves as a valuable tool for monitoring rumen microbial functions and host-microbe interactions.
  • Understanding these relationships can lead to improved strategies for ruminant nutrition and health.