Related Experiment Video

Updated: Mar 30, 2026

An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines
07:18

An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines

Published on: October 11, 2022

2.6K

Electrotransformation and Clonal Isolation of Rickettsia Species

Sean P Riley1, Kevin R Macaluso1, Juan J Martinez1

  • 1Vector-Borne Disease Laboratories, Department of Pathobiological Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana.

Current Protocols in Microbiology
|November 4, 2015
PubMed

Keywords:
cell sortingclonal isolationimmunofluorescenceintracellular bacterialimiting dilutionplaque assaytransformation

More Related Videos

Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae07:10

Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae

Published on: October 8, 2013

79.6K
Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi09:01

Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi

Published on: September 28, 2022

2.8K

Related Concept Videos

Bacterial Phylum Chlamydiae01:29

Bacterial Phylum Chlamydiae

The phylum Chlamydiae or Chlamydiota is composed of a single order, Chlamydiales. This phylum consists entirely of obligate intracellular parasites that infect eukaryotic hosts. While human pathogens within this group have been studied extensively, the phylum encompasses many species capable of interacting with various eukaryotic organisms. Members of Chlamydiae are typically small cocci, approximately 0.5 μm in diameter, and exhibit a distinctive developmental cycle. As is characteristic...
702
Intracellular Movement of Viruses and Bacteria01:10

Intracellular Movement of Viruses and Bacteria

Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
3.8K