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Activation effect of Ganoderma lucidum polysaccharides liposomes on murine peritoneal macrophages
Zhenguang Liu1, Jie Xing1, Yee Huang1
1Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing 210095, PR China.
Abstract:
The activation of murine peritoneal macrophages by Ganoderma lucidum polysaccharides liposomes (GLPL) was investigated in vitro. After treatment with GLPL, the changes of the nitric oxide (NO) secretion and iNOS (inducible nitric oxide synthase) activity were evaluated. The results showed that NO production and iNOS activity of macrophages were enhanced compared to GLP and BL group. In addition, both the phagocytic activity and levels of cytokines IL-1β, TNF-α and IFN-γ were enhanced in the peritoneal macrophages of mice by stimulation of GLPL. The expression of the major histocompatibility complex class II molecule (MHC II) on the surface of peritoneal macrophages significantly increased. These indicated that GLPL could enhance the activation of peritoneal macrophages and their potential for use as a delivery system of GLP.
Insights
Ganoderma lucidum polysaccharides liposomes (GLPL) effectively activate murine peritoneal macrophages. This activation boosts nitric oxide secretion, cytokine production, and phagocytic activity, suggesting GLPL
Area of Science:
- Immunology
- Pharmacology
Background:
- Ganoderma lucidum polysaccharides (GLP) are known for their immunomodulatory effects.
- Liposomal delivery systems can enhance the bioavailability and efficacy of active compounds.
Purpose of the Study:
- To investigate the in vitro activation of murine peritoneal macrophages by Ganoderma lucidum polysaccharides liposomes (GLPL).
- To evaluate the potential of GLPL as a delivery system for GLP.
Main Methods:
- Murine peritoneal macrophages were treated with GLPL.
- Nitric oxide (NO) secretion and inducible nitric oxide synthase (iNOS) activity were measured.
- Phagocytic activity and levels of cytokines (IL-1β, TNF-α, IFN-γ) were assessed.
- Expression of MHC class II molecules was analyzed.
Main Results:
- GLPL significantly enhanced NO production and iNOS activity compared to GLP and blank liposomes (BL).
- Phagocytic activity and levels of IL-1β, TNF-α, and IFN-γ were increased in macrophages treated with GLPL.
- GLPL treatment led to a significant increase in MHC class II expression on macrophage surfaces.
Conclusions:
- GLPL effectively activates murine peritoneal macrophages in vitro.
- GLPL demonstrates potential as an effective delivery system for Ganoderma lucidum polysaccharides, enhancing macrophage immune responses.
