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In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
Published on: December 30, 2016
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Functional Modulation of Regulatory T Cells by IL-2
Byung-In Moon1, Tae Hun Kim2, Ju-Young Seoh3
1Department of Surgery, Ewha Womans University Graduate School of Medicine, Seoul, Korea.
Plos One
|November 4, 2015
Summary
Regulatory T cells (Tregs) identification using CD4+CD25+ or CD4+FoxP3+ markers showed similar functions. Interleukin-2 (IL-2) concentration modulated Treg suppressive activity, with low doses enhancing and high doses inhibiting it.
Area of Science:
- Immunology
- Cell Biology
Background:
- Regulatory T cells (Tregs) are crucial for immune homeostasis.
- Phenotypic markers like CD4+CD25+ and FoxP3 are used to identify Tregs.
- Intracellular FoxP3 staining complicates live cell isolation for functional assays.
Purpose of the Study:
- To compare the functional characteristics of CD4+CD25+ and CD4+FoxP3+ Treg fractions.
- To investigate the role of varying Interleukin-2 (IL-2) concentrations on Treg proliferation and suppression.
Main Methods:
- Isolation of CD4+FoxP3+ and CD4+CD25+ fractions from Foxp3 knock-in mice.
- Assessment of proliferative and suppressive activities of isolated Treg fractions.
- Evaluation of Treg function in the presence or absence of varying IL-2 concentrations.
Main Results:
- Both CD4+CD25+ and CD4+FoxP3+ fractions exhibited comparable proliferative and suppressive patterns.
- The CD4+FoxP3+ fraction did not show autocrine proliferation, unlike the CD4+CD25+ fraction.
- Exogenous IL-2 increased the proliferative responsiveness of both Treg fractions compared to responder cells.
- Low-dose IL-2 enhanced Treg suppressive activity, while high-dose IL-2 abrogated it.
Conclusions:
- Phenotypic markers CD4+CD25+ and CD4+FoxP3+ isolate functionally similar Treg populations.
- IL-2 concentration significantly impacts Treg proliferation and suppressive function.
- Understanding these nuances aids in precise Treg isolation and functional assessment for immunotherapy.
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