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Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens
08:23

Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens

Published on: March 10, 2020

[Isolation of Campylobacter jejuni and Campylobacter coli from children with acute intestinal diseases]

T B Safonova, T S Spirina, L A Taranenko

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |January 1, 1989
    PubMed

    Insights

    This study examined 338 children with diarrhea, identifying Campylobacter jejuni and coli in 10% of cases. Microscopic fecal examination proved effective for Campylobacter isolation, influenced by child age and season.

    Area of Science:

    • Pediatrics
    • Microbiology
    • Infectious Diseases

    Background:

    • Diarrhea is a common illness in children.
    • Campylobacter species are known causes of gastroenteritis.

    Purpose of the Study:

    • To investigate the prevalence and etiological role of Campylobacter in childhood diarrhea.
    • To evaluate microscopic examination of native feces for Campylobacter isolation.

    Main Methods:

    • Examination of 338 children (5 days to 14 years) with diarrhea.
    • Sample collection, storage, and inoculation protocols described.
    • Microscopic examination of native fecal samples for Campylobacter.

    Main Results:

    • Isolation of 85 strains: 85 Campylobacter jejuni and Campylobacter coli.
    • Campylobacter isolation rates varied with children's age and season.
    • Microscopic fecal examination demonstrated feasibility for Campylobacter detection.

    Conclusions:

    • Campylobacter is an important etiological agent in 10% of childhood acute enterocolitis and gastroenteritis cases.
    • Microscopic examination of native feces is a viable diagnostic method.
    • Age and season are significant factors in Campylobacter isolation.

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