Fertilization of Mouse Gametes in Vitro Using a Digital Microfluidic System

Area of Science:

• Biotechnology
• Reproductive Science
• Microfluidics

Background:

• In vitro fertilization (IVF) is a key assisted reproductive technology.
• Current IVF methods face challenges in precise manipulation and dynamic culture.
• Digital microfluidic (DMF) systems offer potential for advanced cell manipulation.

Purpose of the Study:

• To evaluate the efficacy and biocompatibility of a DMF system for IVF and embryo culture.
• To assess the impact of sperm concentration on fertilization and embryo viability.
• To demonstrate dynamic embryo culture capabilities using DMF technology.

Main Methods:

• Utilized an electrowetting on dielectric (EWOD) based DMF device for handling gametes and embryos.
• Performed in vitro fertilization (IVF) with mouse gametes on the DMF platform.
• Employed time-lapse microscopy for continuous monitoring of embryo development.
• Investigated the effect of varying sperm concentrations on fertilization rates and embryo survival.

Main Results:

• The DMF device proved biocompatible, with droplet manipulation being harmless to embryos.
• Increasing sperm numbers for IVF enhanced fertilization rates, but excess sperm led to embryo mortality.
• The DMF system successfully supported dynamic culture of mouse embryos to the eight-cell stage.
• Achieved a 34.8% fertilization rate and 25% development to the eight-cell stage using the DMF system.

Conclusions:

• The DMF system, leveraging EWOD, is a viable platform for in vitro fertilization.
• Optimizing sperm concentration is crucial for successful IVF and embryo development.
• This DMF technology holds significant potential for advancing assisted reproductive technologies.