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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

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Published on: February 28, 2015

Analysis of In Vitro Aptamer Selection Parameters.

Maureen McKeague¹, Erin M McConnell¹, Jose Cruz-Toledo²

¹Chemistry Department, Carleton University, 1125 Colonel By Drive, Ottawa, ON, K1S 5B6, Canada.

Journal of Molecular Evolution

|November 5, 2015

Summary

This study analyzed over 492 aptamer selection experiments to identify key factors for successful aptamer discovery. Target choice and selection template significantly impact aptamer development, highlighting the need for better SELEX documentation.

Keywords:

Aptamer Aptamer target Database In vitro selection SELEX

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Last Updated: Mar 30, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

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Published on: February 28, 2015

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Primer-Free Aptamer Selection Using A Random DNA Library

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Published on: July 26, 2010

Area of Science:

Biotechnology
Molecular Biology
Biochemistry

Background:

Nucleic acid aptamers offer advantages over antibodies and peptides for molecular recognition.
Challenges in aptamer selection, characterization, and validation hinder their use in diagnostics and therapeutics.

Purpose of the Study:

To perform the first parameter analysis of conditions used in de novo aptamer selection.
To identify factors influencing the success of aptamer discovery through systematic review of published experiments.

Main Methods:

Extracted detailed information from 492 published SELEX (Systematic Evolution of Ligands Exponential Enrichment) experiments.
Analyzed relationships between nucleic acid library, target choice, selection methods, experimental conditions, and aptamer affinity.

Main Results:

The choice of target and selection template had the most significant impact on the success of de novo aptamer selection.
Identified key parameters influencing aptamer candidate affinity and isolation.

Conclusions:

Emphasize the need for improved documentation and thorough experimentation of SELEX criteria for better correlation with selection success.
Findings provide insights for optimizing aptamer selection strategies to enhance diagnostic and therapeutic applications.