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Skin Tattooing As A Novel Approach For DNA Vaccine Delivery
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Imaging Functional Nucleic Acid Delivery to Skin.

Roger L Kaspar1, Robyn P Hickerson2, Emilio González-González3

  • 1TransDerm Inc., 2161 Delaware Ave, Santa Cruz, CA, 95060, USA. Roger.Kaspar@TransDermInc.com.

Methods in Molecular Biology (Clifton, N.J.)
|November 5, 2015
PubMed
Summary

Developing nucleic acid therapies for monogenic skin diseases faces delivery challenges due to skin barriers. Combining topical delivery methods with advanced imaging is key for effective treatment and monitoring.

Keywords:
BioluminescenceBiophotonicFluorescenceGFPGene therapyKeratinMonogenicNucleic acidOptical imagingPachyonychia congenitaSkinTopical deliverysiRNA

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Area of Science:

  • Dermatology and Genetic Medicine
  • Biotechnology and Nucleic Acid Therapeutics
  • Medical Imaging and Diagnostics

Background:

  • Monogenic skin diseases are caused by single gene mutations, making them prime candidates for nucleic acid-based therapies.
  • The skin's superficial accessibility is advantageous, yet significant barriers hinder nucleic acid delivery to target cells.
  • Overcoming the stratum corneum, skin's layered structure, and cellular membranes is crucial for therapeutic success.

Purpose of the Study:

  • To review strategies for overcoming skin barriers for nucleic acid delivery.
  • To highlight the role of imaging technologies in monitoring nucleic acid delivery and therapeutic efficacy.
  • To discuss the development of nucleic acid-based therapies for treating skin diseases.

Main Methods:

  • Evaluation of chemical, physical, and electrical topical delivery methods.
  • Utilizing a range of optical imaging modalities for real-time monitoring.
  • Employing animal models and reporter genes to assess delivery and expression.

Main Results:

  • Combinations of delivery approaches are necessary to penetrate skin barriers and reach intracellular targets.
  • Imaging tools significantly enable the development and testing of nucleic acid delivery systems.
  • Successful delivery allows for local gene expression or gene silencing for therapeutic purposes.

Conclusions:

  • Effective nucleic acid delivery to skin cells requires overcoming multiple biological barriers.
  • Advanced imaging techniques are indispensable for guiding and validating topical nucleic acid therapies.
  • This research paves the way for novel nucleic acid-based treatments for various skin disorders.