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Synthesis of radiolabeled acetyl-coenzyme A from sodium acetate
R C Clough1, S R Barnum, J G Jaworski
1Department of Botany, Miami University, Oxford, Ohio 45056.
Analytical Biochemistry
|January 1, 1989
Abstract:
The synthesis of high specific radioactivity [14C]-acetyl-Coenzyme A from [14C]sodium acetate, 2,6-dichlorobenzoic acid, 1,1'-carbonyldiimidazole, and CoA is reported. Starting with 1 mumol of [14C]sodium acetate, this method yields pure [14C]acetyl-CoA in yields approaching 40%. Chromatography on a reversed-phase ODS column was used to separate acetyl-CoA from Coenzyme A and side products. The acetylating agent is apparently a reaction intermediate, acetylimidazole.