Related Experiment Video
Updated: Mar 30, 2026
03:55
A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
Published on: June 27, 2022
4.4K
H2S, a novel gasotransmitter, involves in gastric accommodation
Ailin Xiao1, Hongjuan Wang2, Xin Lu1
1Department of Physiology, Shandong University School of Medicine, Jinan, People's Republic of China.
Scientific Reports
|November 5, 2015
Summary
Endogenous hydrogen sulfide (H2S), produced by cystathionine-β-synthase (CBS), plays a key role in gastric accommodation. Reduced CBS activity impairs gastric compliance and is linked to functional dyspepsia.
Area of Science:
- Gastroenterology
- Biochemistry
- Physiology
Background:
- Hydrogen sulfide (H2S) is primarily synthesized by cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE) from L-cysteine.
- H2S is recognized as a crucial gasotransmitter involved in various physiological processes.
Purpose of the Study:
- To investigate the role of endogenous H2S in regulating gastric accommodation.
- To determine the specific enzyme, CBS or CSE, responsible for H2S production in gastric accommodation.
Main Methods:
- Utilized CBS(+/-) mice, immunohistochemistry, immunoblotting, methylene blue assay, intragastric pressure recording, and electrical field stimulation (EFS).
- Administered H2S donors (NaHS, L-Cys) and enzyme inhibitors (AOAA for CBS, PAG for CSE).
- Assessed gastric compliance, fundus strip relaxation, and enzyme/protein expression levels post-feeding.
Main Results:
- Mouse gastric fundus expresses both CBS and CSE, producing H2S.
- H2S donors (L-Cys) increased gastric compliance and enhanced non-adrenergic, non-cholinergic (NANC) relaxation.
- CBS inhibition (AOAA) and CBS deficiency (CBS(+/-) mice) significantly reduced gastric compliance.
- CSE inhibition (PAG) had no effect on gastric compliance.
- CBS expression and H2S production increased post-feeding.
- Metabolic aberrations of H2S were observed in patients with functional dyspepsia (FD).
Conclusions:
- Endogenous H2S, primarily mediated by CBS, is a critical regulator of gastric accommodation.
- Dysregulation of H2S metabolism may contribute to functional dyspepsia.
Related Concept Videos
01:28Acid Suppressive Drugs for Peptic Ulcer Disease: Histamine H2-Receptor Antagonists
1.2K
Histamine H2 receptors, which are intricately located on the basolateral membrane of parietal cells, play a crucial role in modulating gastric acid secretion. When released from enterochromaffin-like cells, histamine engages H2 receptors, initiating the cyclic AMP (cAMP) pathway. In this pathway, adenylyl cyclase converts ATP into cAMP, elevating intracellular cAMP levels. The activation of protein kinase A follows, stimulating the proton pump. This stimulation prompts the secretion of hydrogen...
1.2K
01:26Gastric Phase of Digestion
4.3K
The gastric phase of digestion begins as soon as food enters the stomach. The incoming food bolus triggers neural and hormonal mechanisms, which last approximately 3 to 4 hours. During this phase, the stomach undergoes significant changes to prepare the food for further digestion and absorption.
When food enters the stomach, it stretches the stomach walls and activates stretch receptors. This triggers local reflexes of the enteric nervous system, mediated through the myenteric plexus. These...
When food enters the stomach, it stretches the stomach walls and activates stretch receptors. This triggers local reflexes of the enteric nervous system, mediated through the myenteric plexus. These...
4.3K
01:21Stomach pH Regulation
7.7K
The human body carefully regulates the internal pH of different organs to maintain homeostasis. For example, while the blood plasma maintains a neutral pH of 7, the stomach lumen has an acidic pH of 1.5 - 3.5. The low pH of stomach lumen helps kill pathogens in the food and break down complex food molecules.
The acid-secreting gastric mucosal epithelial cells (parietal cells) lining the stomach lumen maintain the low pH in the lumen. Numerous ion transporters and channels on these parietal...
The acid-secreting gastric mucosal epithelial cells (parietal cells) lining the stomach lumen maintain the low pH in the lumen. Numerous ion transporters and channels on these parietal...
7.7K
01:29Intestinal Phase of Digestion
12.2K
The intestinal phase of digestion is the third and final stage of the digestive process, occurring after the cephalic and gastric phases. It begins when chyme, a partially digested mixture of food and digestive enzymes, enters the small intestine from the stomach. This phase is crucial for nutrient absorption and involves complex hormonal and enzymatic interactions.
The arrival of the chyme in the small intestine distends the duodenum, which triggers the enterogastric reflex. This distension...
The arrival of the chyme in the small intestine distends the duodenum, which triggers the enterogastric reflex. This distension...
12.2K
01:23Drugs Affecting GI Tract Motility: Serotonin Receptor Agonists
1.2K
Serotonin, a crucial neurotransmitter synthesized by enterochromaffin cells, plays a cardinal role in regulating gastrointestinal (GI) motility. With over 90% of the body's total serotonin in the GI tract, its influence on digestive processes is profound. Serotonin is swiftly released upon various stimuli, such as food boluses or certain drugs, triggering intrinsic sensory neurons in the myenteric plexus and extrinsic vagal and spinal sensory neurons. This leads to the activation of the...
1.2K
01:40Hormonal Regulation
50.5K
Hormones regulate a significant portion of digestion through activation of the neuroendocrine system. The neuroendocrine system of digestion contains many different hormones all with multiple functions that are both, directly and indirectly, involved in digestion.
50.5K