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Related Experiment Videos

Epidermal growth factor and the kidney.

D A Fisher1, E C Salido, L Barajas

  • 1Department of Pediatrics, University of California, Los Angeles School of Medicine 90509.

Annual Review of Physiology
|January 1, 1989
PubMed
Summary

The mammalian kidney synthesizes epidermal growth factor (EGF), with prepro EGF mRNA found in kidney tubules. Urinary EGF peptides may maintain the integrity of the urinary tract lining.

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Area of Science:

  • Nephrology
  • Molecular Biology
  • Biochemistry

Background:

  • The mammalian kidney is a key site for epidermal growth factor (EGF) synthesis, second only to the salivary gland in rodents.
  • Prepro EGF mRNA is specifically localized to the thick ascending limb of Henle (TALH) and distal convoluted tubule (DCT) cells in the kidney.

Purpose of the Study:

  • To investigate the presence, processing, and potential function of epidermal growth factor (EGF) within the mammalian kidney.
  • To characterize the molecular forms of EGF found in kidney tissue and urine.
  • To explore the role of EGF in renal tubular cells and its potential contribution to renal hypertrophy and function.

Main Methods:

  • Localization of prepro EGF mRNA in kidney tissues.
  • Analysis of molecular mass and peptide composition of EGF in kidney and urine.
  • Immunoelectron microscopy to identify EGF immunoreactivity in renal cells.
  • Assessment of EGF receptor presence and response in renal tubular cells.

Main Results:

  • Kidney prepro EGF mRNA is found in TALH and DCT cells, with similar transcripts to salivary gland EGF.
  • Several EGF peptides, including 6kDa and 30kDa species, are excreted in urine.
  • EGF immunoreactivity is localized to the apical plasma membrane of TALH and DCT cells.
  • Renal tubular cells possess EGF receptors and respond to EGF with increased DNA synthesis and mitogenesis.
  • Prepro EGF mRNA levels increase in TALH and DCT cells during renal hypertrophy.

Conclusions:

  • The kidney actively synthesizes and processes EGF, with specific forms secreted into urine.
  • Urinary EGF may play a role in maintaining the integrity of the urinary tract epithelium.
  • EGF signaling in renal tubules, potentially through membrane-bound forms, may influence tubular function and response to injury, such as hypertrophy.

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