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Published on: July 16, 2018
Nanoparticle-assisted laser desorption/ionization using sinapic acid-modified iron oxide nanoparticles for mass
Hanaka Komori1, Riho Hashizaki1, Issey Osaka2
1Department of Bioscience, Fukui Prefectural University, Eiheiji, Fukui 910-1195, Japan. staira@fpu.ac.jp.
Abstract:
Iron oxide-based nanoparticles (NP) were covalently modified with sinapic acid (SA) through a condensation reaction to assist the ionization of both large and small molecules. The morphology of SA-modified NPs (SA-NP) was characterized by transmission electron microscopy (TEM), and the modification of the NP surface with SA was confirmed using ultraviolet (UV) and infrared (IR) spectroscopy. The number of SA molecules was estimated to be 6 per NP. SA-NP-assisted laser desorption/ionization was carried out on small molecules, such as pesticides and plant hormones, and large molecules, such as peptides and proteins. A peptide fragment from degraded proteins was detected more efficiently compared with conventional methods.
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