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Updated: Mar 30, 2026
Mouse Models Of Helicobacter Infection And Gastric Pathologies
Published on: October 18, 2018
Expression of macrophage migration-inhibitory factor in duodenal ulcer and its relation to Helicobacter pylori
1Department of Gastroenterology, General Hospital of Lanzhou Military Command, Lanzhou, Gansu Province, China yuxiaohui_l@163.com.
Abstract:
The aim of this study was to examine the expression of macrophage migration-inhibitory factor (MIF) in duodenal ulcer epithelial cells and its relation to Helicobacter pylori (Hp) infection, and to discuss the pathogenic roles of MIF expression and Hp infection in duodenal ulcer. MIF protein and mRNA expression was examined in samples from patients with duodenal ulcer with and without Hp infection (N = 40 each, experimental group), and in normal duodenal bulb mucosal tissue (N = 40, control group) using immunohistochemistry and in situ hybridization. Patients without Hp infection received routine treatment, and treatment was provided to the patients positive for Hp to eradicate Hp infection. Hp and MIF expression levels before treatment and after the ulcer had been cured were compared. The positive rates of MIF protein and mRNA in patients with Hp infection before treatment were 67.5 and 65%, respectively, and were 18.9 and 21.6% in the 37 patients from whom Hp was eliminated. These were statistically different both before and after treatment compared with controls (P < 0.05). In the patients without Hp infection, the positive rates of MIF protein and mRNA expression before (45 and 47.5%, respectively) and after (32.5 and 30%) treatment were not significantly different (P > 0.05). The results of this study suggested that MIF is related to the development of duodenal ulcer, and that the presence of Hp is closely related with the expression of MIF in the duodenal mucosa and the development of duodenal ulcer.
Insights
Macrophage migration-inhibitory factor (MIF) expression is elevated in duodenal ulcers, particularly with Helicobacter pylori (Hp) infection. Eradicating Hp infection significantly reduces MIF levels, suggesting a role in ulcer pathogenesis.
Area of Science:
- Gastroenterology
- Immunology
- Microbiology
Background:
- Duodenal ulcers are a significant gastrointestinal condition.
- Helicobacter pylori (Hp) infection is a primary cause of duodenal ulcers.
- The role of macrophage migration-inhibitory factor (MIF) in duodenal ulcer pathogenesis requires further elucidation.
Purpose of the Study:
- To investigate macrophage migration-inhibitory factor (MIF) expression in duodenal ulcer epithelial cells.
- To determine the relationship between MIF expression and Helicobacter pylori (Hp) infection in duodenal ulcers.
- To explore the pathogenic roles of MIF and Hp infection in duodenal ulcer development.
Main Methods:
- Immunohistochemistry and in situ hybridization were used to assess MIF protein and mRNA expression.
- Samples were analyzed from duodenal ulcer patients with and without Hp infection, and from healthy controls.
- MIF and Hp expression levels were compared before and after Hp eradication therapy.
Main Results:
- MIF protein and mRNA were significantly upregulated in Hp-infected duodenal ulcer patients compared to controls.
- Successful Hp eradication led to a significant decrease in MIF expression.
- MIF expression in non-Hp-infected duodenal ulcers showed no significant changes after treatment.
Conclusions:
- MIF expression is closely associated with the development of duodenal ulcers.
- Helicobacter pylori infection significantly influences MIF expression in the duodenal mucosa.
- Targeting MIF and Hp may offer therapeutic strategies for duodenal ulcers.
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