Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Significance of Membrane Transport01:44

The Significance of Membrane Transport

44.5K
The transport of solutes across the cell membrane is essential for metabolic processes, like maintaining cell size and volume, generating the action potential, exchanging nutrients and gases, etc. Membrane transport can be either passive or active. It can be simple diffusion, facilitated, or mediated transport aided by transport proteins such as transporters and channels.
Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...
44.5K
Hepatic Drug Clearance: Role of Transporters01:14

Hepatic Drug Clearance: Role of Transporters

369
In the liver and bile canaliculi, influx and efflux transporters modification can influence intrinsic clearance. Transporters play a significant role in moving drugs within liver cells. Elaborate models, such as the Biopharmaceutical Classification System (BCS), are essential to relate transporters to drug disposition. This system categorizes drugs into four classes based on solubility and permeability, providing insights into elimination routes and the effects of transporters following oral...
369
Carrier-Mediated Transport01:06

Carrier-Mediated Transport

1.6K
Carrier-mediated transport is a pivotal process in drug absorption, particularly for lipid-insoluble drugs, and encompasses facilitated diffusion and active transport. Facilitated diffusion allows drugs to move along their concentration gradient without energy expenditure, while active transport utilizes ATP to drive drug movement against this gradient.
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...
1.6K
Electron Transport Chain: Complex III and IV01:43

Electron Transport Chain: Complex III and IV

9.7K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
9.7K
Physiological Pharmacokinetic Models: Incorporating Hepatic Transporter-Mediated Clearance01:07

Physiological Pharmacokinetic Models: Incorporating Hepatic Transporter-Mediated Clearance

372
Drug transporters are critical in drug absorption, distribution, and excretion processes. They should be included in physiological-based pharmacokinetic (PBPK) models, which help predict human drug disposition. However, predicting this is challenging during drug development, especially when liver transport is involved. However, with a realistic representation of body transport processes, an accurate model may be possible.
A recent model describes pravastatin's hepatobiliary excretion,...
372
Pharmacogenetics of Drug Transporters: P-Glycoprotein and Solute Carrier Transporters01:16

Pharmacogenetics of Drug Transporters: P-Glycoprotein and Solute Carrier Transporters

123
The pharmacogenetics of drug transporters is increasingly recognized as a critical factor influencing interindividual variability in drug absorption, distribution, and elimination. These membrane-bound proteins regulate drugs' movement across cellular barriers by actively pumping them out (efflux) or facilitating their uptake (influx). Among the major transporter families, ATP-binding cassette (ABC) and solute carrier (SLC) transporters play particularly prominent roles. Genetic polymorphisms...
123

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Heat shock protein 10 as a chaperone modulating α-synuclein amyloid fibril formation.

Protein science : a publication of the Protein Society·2026
Same author

CAPIM: Catalytic activity and site prediction and analysis tool in multimer proteins.

Protein science : a publication of the Protein Society·2025
Same author

Metal ions control amyloid catalysis.

Journal of inorganic biochemistry·2025
Same author

ATP Hydrolysis by α-Synuclein Amyloids is Mediated by Enclosing β-Strand.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

In silico identification of substrate-binding sites in type-1A α-synuclein amyloids.

Biophysical journal·2025
Same author

Biological Amyloids Chemically Damage DNA.

ACS chemical neuroscience·2025

Related Experiment Video

Updated: Mar 30, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
14:44

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

10.2K

Unresolved questions in human copper pump mechanisms.

Pernilla Wittung-Stafshede1

  • 1Chemistry Department,Umeå University,90187 Umeå,Sweden.

Quarterly Reviews of Biophysics
|November 6, 2015
PubMed
Summary

Cells use copper (Cu) for essential enzymes, but how the ATP7A/B pump transports Cu remains unclear. Understanding this mechanism is vital for fundamental biology and treating diseases linked to copper transport issues.

Keywords:
Menke's disease proteinWilson disease proteinbiophysical methodsceruloplasmincopper transportcopperchaperone

More Related Videos

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
11:04

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides

Published on: September 7, 2019

10.0K
Positron Emission Tomography Using 64-Copper as a Tracer for the Study of Copper-Related Disorders
06:52

Positron Emission Tomography Using 64-Copper as a Tracer for the Study of Copper-Related Disorders

Published on: April 28, 2023

2.1K

Related Experiment Videos

Last Updated: Mar 30, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
14:44

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

10.2K
Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
11:04

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides

Published on: September 7, 2019

10.0K
Positron Emission Tomography Using 64-Copper as a Tracer for the Study of Copper-Related Disorders
06:52

Positron Emission Tomography Using 64-Copper as a Tracer for the Study of Copper-Related Disorders

Published on: April 28, 2023

2.1K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Biophysics

Background:

  • Copper (Cu) is essential for human enzymes, necessitating precise cellular regulation to prevent toxicity.
  • Cells utilize copper-binding proteins, including the ATP7A/B pumps in the Golgi network, to deliver Cu to enzymes.
  • The copper chaperone Atox1 facilitates Cu transfer from the Ctr1 importer to ATP7A/B.

Purpose of the Study:

  • To highlight unresolved mechanistic and biophysical questions regarding the ATP7A/B copper transport mechanism.
  • To emphasize the fundamental and medical importance of understanding copper transport pathways.

Main Methods:

  • Review of existing biological, genetic, and structural data on copper transport proteins.
  • Identification of knowledge gaps in the mechanistic understanding of ATP7A/B function.
  • Perspective-based analysis of unresolved inquiries.

Main Results:

  • Significant progress has been made in understanding copper transport proteins.
  • Key mechanistic questions persist regarding Cu acquisition by ATP7A/B, its conformational changes, and substrate loading.
  • The precise mechanisms of Cu movement through the membrane and delivery to target proteins remain elusive.

Conclusions:

  • Elucidating the ATP7A/B mechanism is crucial for fundamental biological insights into metal transport.
  • Understanding copper dysregulation is critical for addressing various human diseases.
  • Further research into the biophysical mechanisms of ATP7A/B is warranted.