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Detecting 16S rRNA Methyltransferases in Enterobacteriaceae by Use of Arbekacin
Patrick McGann1, Sarah Chahine2, Darius Okafor2
1Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA patrick.t.mcgann4.ctr@mail.mil.
Abstract:
16S rRNA methyltransferases confer resistance to most aminoglycosides, but discriminating their activity from that of aminoglycoside-modifying enzymes (AMEs) is challenging using phenotypic methods. We demonstrate that arbekacin, an aminoglycoside refractory to most AMEs, can rapidly detect 16S methyltransferase activity in Enterobacteriaceae with high specificity using the standard disk susceptibility test.
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