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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Updated: Mar 30, 2026

Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery

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Published on: January 15, 2018

Multivalent Glycopolymer-Coated Gold Nanoparticles.

Sarah-Jane Richards¹, Caroline I Biggs¹, Matthew I Gibson²

¹Department of Chemistry, The University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, UK.

Methods in Molecular Biology (Clifton, N.J.)

|November 6, 2015

Summary

This summary is machine-generated.

Stable, color-changing gold nanoparticles with carbohydrate coatings detect lectins. This versatile method overcomes instability issues, providing reliable results for biological binding events and lectin detection.

Keywords:

Glycopolymers Gold nanoparticle s Lectins RAFT polymerization

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Area of Science:

Nanotechnology
Biochemistry
Materials Science

Background:

Glycosylated noble metal nanoparticles offer unique colorimetric properties for detecting biological binding events, especially lectins.
Colloidal instability in nanoparticles can lead to inaccurate results (false positives) in biological assays.
Polymeric coatings are crucial for enhancing nanoparticle stability.

Purpose of the Study:

To develop a reliable method for coating gold nanoparticles with well-defined polymers bearing carbohydrate end groups.
To create stable, multivalent nanoparticles that maintain rapid colorimetric responses to lectin binding.
To address the challenge of colloidal instability in nanoparticle-based biosensing.

Main Methods:

Synthesized gold nanoparticles.
Developed well-defined polymers with carbohydrate end groups for nanoparticle coating.
Characterized the stability and binding properties of the coated nanoparticles.
Assessed the colorimetric response to lectin binding.

Main Results:

Achieved stable coating of gold nanoparticles using functionalized polymers.
Demonstrated that the resulting multivalent nanoparticles are colloidally stable.
Confirmed that the nanoparticles retain rapid colorimetric responses upon lectin binding.
Validated the approach as versatile and reliable for probing biological binding events.

Conclusions:

The developed method provides stable, carbohydrate-functionalized gold nanoparticles.
These nanoparticles are suitable for reliable lectin detection due to retained colorimetric response and enhanced stability.
This approach offers a promising tool for biosensing applications involving multivalent interactions.