[Rapid Determination of Seven Fungicides in Citrus Fruits]

A rapid and simple determination method of seven fungicides, thiabendazole (TBZ), pyrimethanil (PYR), o-phenylphenol (OPP), fludioxonil (FLD), azoxystrobin (AZX), imazalil (IMZ) and diphenyl (DP) in citrus fruits by LC-MS and HPLC-FL was developed. The seven fungicides were extracted with acetonitrile from citrus fruits and cleaned up with Z-Sep/C18 cartridges. The LC separation was performed on a phenyl-hexyl column with methanol-acetonitrile-10 mmol/L ammonium formate (10 : 35 : 55) as a mobile phase. The recoveries from citrus fruits fortified with the compounds at the MRLs and at 0.1 μg/g ranged from 85.4 to 106.3% and from 75.8 to 99.7%, respectively. The quantitation limits (S/N=10) were 0.03-0.07 μg/g.