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Synthesis, Characterization and Fluorescence Properties of Zn(II) and Cu(II) Complexes: DNA Binding Study of Zn(II)

Parirokh Lavaee¹, Hossein Eshtiagh-Hosseini², Mohammad Reza Housaindokht¹

¹Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, 917751436, Iran.

Journal of Fluorescence

|November 6, 2015

Abstract:

Zinc(II) and copper(II) complexes containing Schiff base, 2- methoxy-6((E)-(phenylimino) methyl) phenol ligand (HL) were synthesized and characterized by elemental analysis, IR, NMR, and single crystal X-ray diffraction technique. The fluorescence properties and quantum yield of zinc complex were studied. Our data showed that Zn complex could bind to DNA grooves with Kb = 10(4) M(-1). Moreover, Zn complex could successfully be used in staining of DNA following agarose gel electrophoresis. MTT assay showed that Zn complex was not cytotoxic in MCF-7 cell line. Here, we introduce a newly synthesized fluorescence probe that can be used for single and double stranded DNA detection in both solution and agarose gels.

Keywords:

Copper DNA Fluorescence Interaction Schiff base Zinc

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