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Pancreastatin inhibits insulin release from Rin m 5F cells: reversion by pertussis toxin
A M Lorinet1, K Tatemoto, M Laburthe
1INSERM U178, Unité de Recherches sur la Différenciation et al Neuroendocrinologie de Cellules Digestives, Bâtiment INSERM, Villejuif, France.
European Journal of Pharmacology
|February 7, 1989
Abstract:
Pancreastatin inhibited carbachol- but not forskolin- or GIP-stimulated insulin release from Rin m 5F cells. The inhibition induced by pancreastatin was dose-dependent, with an ED50 value of 4 nM, and reached a maximum (50% of inhibition) at 10(-7) M peptide. Pretreatment of cells with pertussis toxin abolished the inhibitory effect of pancreastatin on carbachol-induced insulin release. We suggest that pancreastatin exerts a direct inhibitory control on insulin release through a pertussis toxin-sensitive cAMP-independent pathway.