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Stem Cell Culture01:17

Stem Cell Culture

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Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
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Stem Cell Therapy for Tissue Regeneration01:21

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Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
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Embryonic Stem Cells00:57

Embryonic Stem Cells

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
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Adult Stem Cells01:33

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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Related Experiment Video

Updated: Mar 30, 2026

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
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Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

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Harvesting dental stem cells - Overview.

P M Sunil1, Ramanathan Manikandan2, Muthumurugan3

  • 1Department of Oral and Maxillofacial Pathology, Sree Anjaneya Institute of Dental Sciences, Calicut, Kerela, India.

Journal of Pharmacy & Bioallied Sciences
|November 6, 2015
PubMed
Summary
This summary is machine-generated.

Storing dental stem cells from teeth is crucial for future regenerative medicine. This article details the best practices for collecting and preserving these valuable cells for clinical applications.

Keywords:
Dental stem cell bankingdental pulpdental stem cells

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Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
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Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

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Last Updated: Mar 30, 2026

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
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Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
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Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

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Area of Science:

  • Dentistry
  • Regenerative Medicine
  • Stem Cell Biology

Background:

  • Dental stem cells, sourced from various tooth tissues, are a significant area of research in dentistry.
  • The potential clinical applications of these cells necessitate effective storage methods.

Purpose of the Study:

  • To outline the technical procedures for collecting and preserving dental stem cells.
  • To ensure the viability and future usability of dental stem cells for expansion and clinical applications.

Main Methods:

  • Patient preparation for dental stem cell harvesting.
  • Atraumatic and aseptic tooth removal techniques.
  • Safe transportation and preservation protocols for dental tissues.

Main Results:

  • Established protocols for preparing patients for dental stem cell collection.
  • Demonstrated atraumatic and aseptic methods for tooth extraction.
  • Detailed procedures for safe transport and preservation of dental tissues for stem cell isolation.

Conclusions:

  • Standardized technical aspects are essential for successful dental stem cell collection and preservation.
  • Proper handling ensures the integrity of dental stem cells for future therapeutic use.
  • This article provides a foundational guide for researchers and clinicians in dental stem cell banking.