Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Shape, membrane morphology, and morphodynamic response of metabolically active human mitochondria revealed by scanning ion conductance microscopy.

Beilstein journal of nanotechnology·2025
Same author

The Impact of Ultrashort Pulse Laser Structuring of Metals on In-Vitro Cell Adhesion of Keratinocytes.

Journal of functional biomaterials·2024
Same author

Discrimination between the effects of pulsed electrical stimulation and electrochemically conditioned medium on human osteoblasts.

Journal of biological engineering·2023
Same author

Selective adhesion inhibition and hyaluronan envelope reduction of dermal tumor cells by cold plasma-activated medium.

Cell adhesion & migration·2023
Same author

Synergistic effect of cold gas plasma and experimental drug exposure exhibits skin cancer toxicity in vitro and in vivo.

Journal of advanced research·2023
Same author

Coagulation using organic carbonates opens up a sustainable route towards regenerated cellulose films.

Communications chemistry·2023

Related Experiment Video

Updated: Mar 30, 2026

Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds
07:48

Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds

Published on: July 9, 2021

4.1K

Cellular Nutrition in Complex Three-Dimensional Scaffolds: A Comparison between Experiments and Computer Simulations.

Claudia Bergemann1, Patrick Elter2, Regina Lange3

  • 1Department of Cell Biology, University Medical Center Rostock, Schillingallee 69, 18057 Rostock, Germany.

International Journal of Biomaterials
|November 6, 2015
PubMed
Summary

Optimizing nutrient flow in 3D bone scaffolds is crucial for cell growth. This study found that balancing oxygen supply and shear stress is key to improving cell viability within porous implants.

More Related Videos

Self-reporting Scaffolds for 3-Dimensional Cell Culture
14:49

Self-reporting Scaffolds for 3-Dimensional Cell Culture

Published on: November 7, 2013

13.8K
Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds
07:52

Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds

Published on: June 13, 2018

10.8K

Related Experiment Videos

Last Updated: Mar 30, 2026

Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds
07:48

Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds

Published on: July 9, 2021

4.1K
Self-reporting Scaffolds for 3-Dimensional Cell Culture
14:49

Self-reporting Scaffolds for 3-Dimensional Cell Culture

Published on: November 7, 2013

13.8K
Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds
07:52

Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds

Published on: June 13, 2018

10.8K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • In vitro studies of 3D porous scaffolds for bone cell ingrowth face challenges with cell proliferation and differentiation in scaffold cores.
  • Increasing scaffold volume exacerbates issues with nutrient and oxygen diffusion to inner cells.

Purpose of the Study:

  • To develop and utilize an in vitro perfusion cell culture module for analyzing cells within the interior of 3D scaffolds.
  • To investigate the impact of varying medium flow rates on cell viability inside scaffolds.

Main Methods:

  • Development of an in vitro perfusion cell culture system to control medium flow rates through 3D scaffolds.
  • Measurement of cell viability at different flow rates within the scaffold interior.
  • Comparison of experimental results with finite element method (FEM) simulations predicting local oxygen concentration and shear stress.

Main Results:

  • Local cell viability directly correlates with local oxygen concentration and shear stress within the scaffold.
  • Higher perfusion flow rates improve oxygen supply to the core but increase shear stress on surface cells.
  • Excessive shear stress at high flow rates negatively impacts cell vitality, particularly at the scaffold surface.

Conclusions:

  • Both oxygen supply and shear stress are critical parameters influencing cell viability in 3D scaffolds.
  • An optimal nutrient flow rate must balance adequate oxygenation with minimized detrimental shear stress for effective cell growth.
  • This study provides a framework for optimizing perfusion parameters in tissue engineering scaffolds.