Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tissue Homogenization and Cell Lysis01:32

Tissue Homogenization and Cell Lysis

11.5K
Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...
11.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A plasmid-encoded detoxification system contributing to the survival of <i>Klebsiella oxytoca</i> complex bacteria in the presence of the isothiazolinone biocide MIT/CMIT.

Applied and environmental microbiology·2026
Same author

Kitchen Sponges as Reservoirs of Foodborne Pathogens: Microbial Growth Dynamics, Surface Cross-Contamination, and Hygiene Implications.

Journal of food protection·2026
Same author

A framework for the recursive risk-ranking of foodborne zoonotic threats along food supply chains.

One health (Amsterdam, Netherlands)·2026
Same author

Antimicrobial resistance profiles and whole-genome sequence analysis of extended-spectrum β-lactamase (ESBL) production in commensal Escherichia coli from poultry in Türkiye.

PloS one·2026
Same author

Identification of Receptor Binding Proteins of <i>Yersinia</i> Phage φR1-37 and Enterocoliticin That Use the Same Bacterial Surface Receptor.

Viruses·2026
Same author

Editorial: Reviews in molecular evolution of infectious agents and diseases.

Frontiers in microbiology·2026

Related Experiment Video

Updated: Mar 30, 2026

Author Spotlight: Development of an Enhanced Protocol for Rapid and Accurate Isolation of Campylobacter from Food Products
05:34

Author Spotlight: Development of an Enhanced Protocol for Rapid and Accurate Isolation of Campylobacter from Food Products

Published on: February 23, 2024

3.0K

Sampling and Homogenization Strategies Significantly Influence the Detection of Foodborne Pathogens in Meat.

Alexander Rohde1, Jens Andre Hammerl2, Bernd Appel2

  • 1Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany ; Department of Biology, Chemistry and Pharmacy, Free University Berlin, Takustraße 3, 14195 Berlin, Germany.

Biomed Research International
|November 6, 2015
PubMed
Summary

Efficient sample preparation is crucial for food safety testing. For inner-matrix contamination, the FastPrep-24 mill is best, while surface contamination allows more flexibility in homogenization methods.

More Related Videos

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
06:12

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Published on: October 25, 2018

9.3K
Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products
08:19

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products

Published on: January 31, 2025

1.1K

Related Experiment Videos

Last Updated: Mar 30, 2026

Author Spotlight: Development of an Enhanced Protocol for Rapid and Accurate Isolation of Campylobacter from Food Products
05:34

Author Spotlight: Development of an Enhanced Protocol for Rapid and Accurate Isolation of Campylobacter from Food Products

Published on: February 23, 2024

3.0K
Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
06:12

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Published on: October 25, 2018

9.3K
Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products
08:19

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products

Published on: January 31, 2025

1.1K

Area of Science:

  • Food Microbiology
  • Food Safety Analysis
  • Microbiological Testing

Background:

  • Efficient sample preparation, including sampling and homogenization, is vital for accurate food microbiological testing.
  • This critical step is often overlooked in foodstuff control.
  • Standardized methods are needed to ensure reliable results.

Purpose of the Study:

  • To systematically compare different homogenization techniques for food samples.
  • To evaluate their effectiveness for both surface and inner-matrix contamination.
  • To assess the impact of homogenization on pathogen recovery rates and distribution.

Main Methods:

  • Comparison of stomaching, sonication, and milling (FastPrep-24, SpeedMill) for sample homogenization.
  • Use of Salmonella enterica-spiked chicken breasts (surface contamination) and salami/meat paste (inner-matrix contamination).
  • Analysis of pathogen distribution within different sample regions.

Main Results:

  • For surface contamination, various homogenization methods are suitable with minimal loss of culturability.
  • Inner-matrix contamination requires longer homogenization times (up to 8 min), with FastPrep-24 yielding superior recovery rates.
  • Pathogen distribution is not always homogeneous, with higher concentrations found in specific regions (e.g., core of meat paste).

Conclusions:

  • Homogenization method choice significantly impacts pathogen recovery, especially for inner-matrix contamination.
  • Sampling strategies must account for potential non-uniform pathogen distribution.
  • Improving sampling and homogenization techniques is essential for enhancing food safety monitoring.