Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

109
Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
109
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

19.0K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
19.0K
Experimental RNAi02:15

Experimental RNAi

8.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ion valency-driven control of network architecture and tissue-level drug transport in protein-derived hydrogels.

International journal of biological macromolecules·2026
Same author

An Automated Fluorescence Microscopy-Based Sensing System for Continuous Detection of Airborne Asbestos Fibers on a PM<sub>2.5</sub> Monitoring Platform.

Sensors (Basel, Switzerland)·2026
Same author

Multi-functional photonic crystals of modular nanosheets.

Nature communications·2026
Same author

Preemptive Surgical Strategies for Impending Tracheo-Innominate Artery Fistula: A Case Series of Three High-Risk Patients.

Annals of vascular diseases·2026
Same author

High Drug Encapsulation Capacity in Cyclodextrin-Based Nanoparticles: Characterization and <i>In Vivo</i> Antitumor Efficacy in Tumor-Bearing Mice.

ACS bio & med chem Au·2026
Same author

Cationic Nanogel Coated Norovirus VLP Nasal Vaccine Induces Neutralizing Mucosal IgA and Serum IgG Antibodies.

Molecular pharmaceutics·2026

Related Experiment Video

Updated: Mar 30, 2026

Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

11.6K

Amylose-Based Cationic Star Polymers for siRNA Delivery.

Tomoki Nishimura1, Kaori Umezaki1, Sada-atsu Mukai1

  • 1Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo, Kyoto 615-8150, Japan ; ERATO Akiyoshi Bio-Nanotransporter Project, JST, Katsura, Nishikyo, Kyoto 615-8150, Japan.

Biomed Research International
|November 6, 2015
PubMed
Summary

A novel cationic glyco-star polymer effectively delivers siRNA, enhancing gene silencing. This amylose-based nanoplatform shows superior cellular uptake and gene silencing compared to linear polymers.

More Related Videos

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

8.1K
Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

11.7K

Related Experiment Videos

Last Updated: Mar 30, 2026

Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

11.6K
Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

8.1K
Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

11.7K

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Polymer Chemistry

Background:

  • Developing efficient and safe siRNA delivery systems is crucial for gene therapy.
  • Cationic polymers are widely explored for nucleic acid complexation and delivery.
  • Star polymers offer unique structural advantages for nanocarrier design.

Purpose of the Study:

  • To synthesize and characterize a novel cationic glyco-star polymer for siRNA delivery.
  • To evaluate the siRNA complexation, cellular uptake, and gene silencing efficacy of the star polymer.
  • To assess the potential of amylose-based star polymers as glycobiomaterials for nanomedicine.

Main Methods:

  • Chemoenzymatic synthesis of spermine-modified 8-arm amylose star polymer.
  • Formation and characterization of siRNA/star polymer complexes (hydrodynamic diameter ~230 nm).
  • In vitro evaluation of cellular uptake and gene silencing efficiency compared to a linear polymer control.

Main Results:

  • The cationic 8-arm star polymer effectively bound siRNA, forming stable spherical complexes.
  • Complexes exhibited significantly enhanced cellular uptake compared to the 1-arm polymer.
  • Superior gene silencing effects were observed with the 8-arm star polymer system.

Conclusions:

  • Amylose-based star polymers represent a promising nanoplatform for siRNA delivery.
  • The star architecture enhances cellular uptake and gene silencing efficacy.
  • This approach holds potential for developing advanced glycobiomaterials in nanomedicine.