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Application of CRISPR/Cas9 Technology to HBV
Guigao Lin1, Kuo Zhang2, Jinming Li3
1National Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, China. linguigao1982@163.com.
International Journal of Molecular Sciences
|November 6, 2015
Summary
The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing system offers a novel therapeutic approach for chronic hepatitis B virus (HBV) infection. This technology targets the persistent viral DNA, showing promise for achieving a sustained viral response and potentially eradicating HBV.
Area of Science:
- Hepatology
- Molecular Biology
- Gene Therapy
Background:
- Over 240 million people globally suffer from chronic hepatitis B virus (HBV) infection.
- Current treatments like nucleos(t)ide analogs and interferon do not eliminate the persistent viral DNA (cccDNA).
- The inability to target cccDNA limits sustained viral response (SVR) and cure rates.
Purpose of the Study:
- To review the potential of the CRISPR/Cas9 gene editing system as a novel therapy for HBV.
- To discuss the application of CRISPR/Cas9 in targeting and suppressing HBV replication.
Main Methods:
- CRISPR/Cas9 system targeting conserved HBV genome regions.
- Review of studies demonstrating the efficacy of CRISPR/Cas9 in HBV suppression.
Main Results:
- CRISPR/Cas9 specifically targets HBV DNA, leading to robust viral suppression.
- The system shows potential for eradicating the virus by targeting its persistent form.
Conclusions:
- CRISPR/Cas9 gene editing represents a promising new strategy for treating chronic HBV.
- This approach addresses the critical need for therapies targeting the viral cccDNA to achieve SVR or cure.
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