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Related Experiment Video

Updated: Mar 30, 2026

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
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Direct Visualization of DNA Replication Dynamics in Zebrafish Cells.

Kenji Kuriya1, Eriko Higashiyama1, Eriko Avşar-Ban2

  • 11 Laboratory of Molecular and Cellular Biology, Department of Life Sciences, Graduate School of Bioresources, Mie University , Tsu, Japan .

Zebrafish
|November 6, 2015
PubMed
Summary

Zebrafish DNA replication starts in the nuclear interior and proceeds to the periphery, similar to mammals. However, interior DNA replication takes longer in zebrafish, suggesting unique genomic organization.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Spatiotemporal regulation of DNA replication is crucial for S-phase progression and is well-studied in mammalian cells.
  • Limited information exists regarding DNA replication dynamics in nonmammalian vertebrates.

Purpose of the Study:

  • To investigate and characterize the spatiotemporal dynamics of DNA replication in zebrafish (Danio rerio) cells.
  • To compare zebrafish replication patterns with those observed in mammalian cells.

Main Methods:

  • Direct visualization of replicating DNA within zebrafish nuclei.
  • Analysis of DNA fiber molecules isolated from zebrafish cells.
  • DNA combing technique to measure replication fork progression and origin-to-origin distances.

Main Results:

  • Zebrafish DNA replication initiates at the nuclear interior and progresses towards the periphery, mirroring mammalian patterns.
  • Interior DNA replication occupies a relatively longer duration in zebrafish compared to mammals.
  • Replication fork progression rate (∼1.4 kb/min) and origin-to-origin distance (∼100 kb) in zebrafish are comparable to mammalian cells.

Conclusions:

  • Zebrafish exhibit conserved spatiotemporal regulation of DNA replication, with notable differences in the duration of interior replication.
  • Observed differences may be attributed to zebrafish-specific genomic organization.
  • This study provides the first quantitative measurement of DNA replication dynamics in zebrafish.