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Updated: Mar 30, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
The complete mitochondrial genome of Triplophysa sp. (Teleostei: Cypriniformes: Balitoridae)
1a Co-Innovation Center for Qinba Regions' Sustainable Development, College of Life Science, Shaanxi Normal University , Xi'an , China.
Abstract:
The complete mitochondrial genome sequence of balitorid fish, Triplophysa sp., which was from Qinling mountains, central China, has been sequenced by the long polymerase chain with primer walking method. The mitochondrial genome of Triplophysa sp. is similar to those of the typical vertebrates, 16 570 bp in length, including 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and one control region (D-loop). Most mitochondrial genes are encoded on the heavy strand, except for ND6 and eight tRNA genes which are encoded on the light strand. The overall base composition of the heavy strand of the Triplophysa sp. mitogenome is A: 28.0%, T: 28.0%, C: 25.8%, and G: 18.2%. Combined with the complete mitogenome sequences of other 15 balitorids, the topology demonstrated that Triplophysa sp. has a close relationship with the cluster consisting of Triplophysa yarkandensis, Triplophysa siluroides, and Triplophysa robusta with high support values. The genera Triplophysa and Barbatula formed a sister-group relationship. Further investigations with more Triplophysa species included need to be performed to better understand the evolutionary history of this intriguing genus.
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