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Updated: Mar 30, 2026

Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
The complete mitochondrial DNA genome of a Chloridea (Heliothis) subflexa (Lepidoptera: Noctuidae) morpho-species
Bruna Raquel de Souza1,2, Wee Tek Tay1, Cecilia Czepak3
1a CSIRO, Black Mountain Laboratories , Canberra , ACT , Australia.
Abstract:
We investigate the complete mitogenome of a pheromone-trapped morpho-species of Chloridea subflexa from Brazil (initially identified by the Sanger sequencing of partial mtCOI gene) as 15 323 bp (KT598688) via next generation sequencing platform. The mitogenome has an A/T rich base composition (A: 40.4%; T: 40.3%; C: 11.5%; G: 7.8%), and included 13 protein-coding genes (PCGs), 22 tRNAs, 2 ribosomal RNAs and a putative replication region (ca. 323 bp). All PCGs start with a methionine (M) amino acid except the COI gene which has an arginine (R). The trnL2 and trn-Lys genes were partially embedded within the COII gene, while the trn-His gene was completely embedded within the ND4 gene. All PCGs ends with the "TAA" stop codon except ND3 which has a "TAG" stop codon.
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