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The effect of sequential sampling on crevicular fluid volume and enzyme activity
I B Lamster1, D S Harper, S Goldstein
1Department of Periodontics and Oral Medicine, Fairleigh S. Dickinson, Jr., College of Dental Medicine.
Journal of Clinical Periodontology
|April 1, 1989
Summary
Static gingival crevicular fluid (GCF) samples show higher enzyme activity than sequential samples. This finding is crucial for understanding GCF constituents in gingivitis and periodontitis.
Area of Science:
- Periodontology
- Biochemistry
- Biomaterials
Background:
- Gingival crevicular fluid (GCF) contains enzymes and other biomarkers relevant to periodontal health.
- Understanding GCF dynamics is essential for diagnosing and monitoring periodontal diseases.
Purpose of the Study:
- To compare the volume and enzyme activity in static GCF samples versus sequential samples collected over 60 minutes.
- To investigate GCF enzyme activity patterns in patients with gingivitis and chronic adult periodontitis.
Main Methods:
- GCF was collected using filter paper strips from interproximal and facial sites in patients with gingivitis and periodontitis.
- Samples were collected at 0, 4, 8, 30, and 60 minutes.
- Microspectrophotometry assessed cytoplasmic and lysosomal enzyme activity.
Main Results:
- GCF volume showed minimal fluctuation with repeated sampling.
- Static GCF samples exhibited higher total enzyme activity compared to subsequent samples.
- Enzyme concentration was generally higher in static samples and facial sites than interproximal sites.
Conclusions:
- Static GCF samples provide a more comprehensive measure of total enzyme activity and concentration.
- GCF enzyme activity patterns differ between gingivitis and periodontitis, particularly at facial sites.
- The findings suggest static GCF collection is valuable for periodontal biomarker assessment.