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Flow cytometry in hematological nonmalignant disorders.
M C Béné1, Y Le Bris1, N Robillard1
1Hematology Biology, University Hospital of Nantes, Nantes, France.
International Journal of Laboratory Hematology
|November 7, 2015
Summary
Multiparameter flow cytometry (MFC) aids in diagnosing blood cancers. This review explores MFC
Area of Science:
- Hematology
- Immunophenotyping
- Clinical Laboratory Science
Background:
- Multiparameter flow cytometry (MFC) is crucial for diagnosing hematological malignancies.
- Its utility extends to nonmalignant and premalignant hematologic disorders.
- Understanding normal leukocyte subset immunophenotypes is essential.
Purpose of the Study:
- To review the applications of MFC beyond malignancy diagnosis.
- To describe normal leukocyte immunophenotypic characteristics and their alterations.
- To highlight MFC's role in specific hematologic conditions.
Main Methods:
- Review of existing literature on MFC applications in hematology.
- Description of normal peripheral blood leukocyte subset immunophenotypes.
- Discussion of MFC marker utility in various clinical scenarios.
Main Results:
- MFC provides insights into normal leukocyte subset profiles.
- Specific markers are valuable for evaluating hyperlymphocytosis, infections, hypereosinophilia, paroxysmal nocturnal hemoglobinuria, and large granular lymphocyte disorders.
- MFC applications are expanding to include red blood cell and platelet analyses.
Conclusions:
- Multiparameter flow cytometry is a versatile tool in hematology laboratories.
- MFC aids in the characterization of various nonmalignant and premalignant hematologic conditions.
- Further developments promise broader applications of MFC in blood cell analysis.
