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Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation
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Flow cytometry in hematological nonmalignant disorders.

M C Béné1, Y Le Bris1, N Robillard1

  • 1Hematology Biology, University Hospital of Nantes, Nantes, France.

International Journal of Laboratory Hematology
|November 7, 2015
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Summary
This summary is machine-generated.

Multiparameter flow cytometry (MFC) aids in diagnosing blood cancers. This review explores MFC

Keywords:
Flow cytometryerythrocytesleukocytesnonmalignant disordersperipheral blood

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Area of Science:

  • Hematology
  • Immunophenotyping
  • Clinical Laboratory Science

Background:

  • Multiparameter flow cytometry (MFC) is crucial for diagnosing hematological malignancies.
  • Its utility extends to nonmalignant and premalignant hematologic disorders.
  • Understanding normal leukocyte subset immunophenotypes is essential.

Purpose of the Study:

  • To review the applications of MFC beyond malignancy diagnosis.
  • To describe normal leukocyte immunophenotypic characteristics and their alterations.
  • To highlight MFC's role in specific hematologic conditions.

Main Methods:

  • Review of existing literature on MFC applications in hematology.
  • Description of normal peripheral blood leukocyte subset immunophenotypes.
  • Discussion of MFC marker utility in various clinical scenarios.

Main Results:

  • MFC provides insights into normal leukocyte subset profiles.
  • Specific markers are valuable for evaluating hyperlymphocytosis, infections, hypereosinophilia, paroxysmal nocturnal hemoglobinuria, and large granular lymphocyte disorders.
  • MFC applications are expanding to include red blood cell and platelet analyses.

Conclusions:

  • Multiparameter flow cytometry is a versatile tool in hematology laboratories.
  • MFC aids in the characterization of various nonmalignant and premalignant hematologic conditions.
  • Further developments promise broader applications of MFC in blood cell analysis.