Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

101.0K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
101.0K
RNA-seq03:21

RNA-seq

12.5K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.5K
Sanger Sequencing01:57

Sanger Sequencing

778.4K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
778.4K
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

653
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
653
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

13.6K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
13.6K
Genomics02:02

Genomics

41.7K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
41.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evolving incidence patterns for locally advanced operable breast cancer by receptor status: SEER 2010-2021.

NPJ breast cancer·2025
Same author

The RNA m<sup>6</sup>A landscape during human oocyte-to-embryo transition.

The EMBO journal·2025
Same author

Improving gene isoform quantification with miniQuant.

Nature biotechnology·2025
Same author

Influenza vaccination during early pregnancy and risk of major birth defects, US Birth Defects Study To Evaluate Pregnancy exposureS, 2014-2019.

Vaccine·2025
Same author

Zygotic activation of transposable elements during zebrafish early embryogenesis.

Nature communications·2025
Same author

Maternal Exposure to Tap Water Disinfection By-Products and Risk of Selected Congenital Heart Defects.

Birth defects research·2024
Same journal

Advancing Functional Transcriptomics in Zebrafish with High-accuracy Full-length RNA Sequencing.

Genomics, proteomics & bioinformatics·2026
Same journal

NanoRAPID: A Deep Learning-based Framework for Single-molecule RNA Structure Analysis Using Nanopore Direct RNA Sequencing.

Genomics, proteomics & bioinformatics·2026
Same journal

Single-cell Multiomic and Spatiotemporal Dissection of the Liver Circadian Clock.

Genomics, proteomics & bioinformatics·2026
Same journal

Ï€-HelixNovo2: Making Accurate Online De Novo Peptide Sequencing Available to All.

Genomics, proteomics & bioinformatics·2026
Same journal

CTSC-RAB38 Potentiates Responsiveness to PD-1 Blockade in Esophageal Squamous Cell Carcinoma.

Genomics, proteomics & bioinformatics·2026
Same journal

Comprehensive Benchmarking with Guidelines for Analyzing Transposable Element-derived RNA Expression.

Genomics, proteomics & bioinformatics·2026
See all related articles

Related Experiment Video

Updated: Mar 30, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

14.9K

PacBio Sequencing and Its Applications.

Anthony Rhoads1, Kin Fai Au2

  • 1Department of Biostatistics, University of Iowa, Iowa City, IA 52242, USA.

Genomics, Proteomics & Bioinformatics
|November 7, 2015
PubMed
Summary
This summary is machine-generated.

Pacific Biosciences (PacBio) single-molecule sequencing provides long reads for genome and transcriptome research. This technology aids in closing genome gaps, identifying structural variations, and discovering novel genes and isoforms.

Keywords:
De novo assemblyGene isoform detectionHybrid sequencingMethylationThird-generation sequencing

More Related Videos

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
06:40

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

1.9K
Amplicon Sequencing using the Long-Read Sequencing Technologies
08:57

Amplicon Sequencing using the Long-Read Sequencing Technologies

Published on: August 29, 2025

639

Related Experiment Videos

Last Updated: Mar 30, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

14.9K
Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
06:40

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

1.9K
Amplicon Sequencing using the Long-Read Sequencing Technologies
08:57

Amplicon Sequencing using the Long-Read Sequencing Technologies

Published on: August 29, 2025

639

Area of Science:

  • Genomics
  • Transcriptomics
  • Epigenetics

Background:

  • Second-generation sequencing (SGS) technologies have limitations in read length.
  • Unsolved problems in genome, transcriptome, and epigenetics research require advanced sequencing capabilities.

Purpose of the Study:

  • To highlight the advantages of PacBio single-molecule, real-time sequencing.
  • To discuss its applications in genome assembly, structural variation detection, and transcriptome analysis.
  • To explore its utility in epigenetics and hybrid sequencing strategies.

Main Methods:

  • Utilizing single-molecule, real-time sequencing technology from Pacific Biosciences.
  • Employing PacBio sequencing for de novo genome assembly and transcriptome analysis.
  • Implementing hybrid sequencing strategies combining PacBio long reads with short reads.

Main Results:

  • PacBio sequencing enables highly contiguous de novo assemblies, closing gaps in reference genomes.
  • Longer reads facilitate the characterization of structural variations and detection of disease-associated mutations.
  • PacBio transcriptome sequencing aids in identifying gene isoforms, novel genes, and novel isoforms.
  • Direct detection of base modifications, such as methylation, is possible.
  • Hybrid strategies offer a more affordable and scalable alternative, especially for smaller labs.

Conclusions:

  • PacBio sequencing significantly advances genome, transcriptome, and epigenetics research beyond SGS capabilities.
  • Its long-read technology is crucial for resolving complex genomic regions and variations.
  • Hybrid approaches enhance scalability and cost-effectiveness for broader accessibility.