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Histomorphometric Analysis of Contaminated Autogenous Tooth Graft Materials After Various Sterilization
Soo-Young Jin1, Su-Gwan Kim, Ji-Su Oh
1*Graduate Student, Department of Oral and Maxillofacial Surgery, College of Dentistry, Chosun University, Gwangju, Republic of Korea. †Professor, Department of Oral and Maxillofacial Surgery, School of Dentistry, Chosun University, Gwangju, Republic of Korea. ‡Associate Professor, Department of Oral and Maxillofacial Surgery, School of Dentistry, Chosun University, Gwangju, Republic of Korea. §Assistant Professor, Department of Oral and Maxillofacial Surgery, School of Dentistry, Chosun University, Gwangju, Republic of Korea. ¶Professor, Department of Pathology, School of Medicine, Chosun University, Gwangju, Republic of Korea. ‖Associate Professor, Department of Dental Hygiene, Kangwon National University, Samcheok, Republic of Korea. #Clinical Doctor, Misomore Dental Clinic, Jeonju, Republic of Korea.
Purpose:
The purpose of this study was to evaluate histomorphometrically contaminated autogenous tooth graft materials, which were resterilized.
Materials And Methods:
The intentional defects (diameter: 8 mm, depth: 4 mm) were formed around implant fixture on the iliac crest of 6 mongrel dogs. Autogenous tooth graft materials were made by extracted premolars. After the contamination of the tooth materials, graft procedure was performed; no contaminated group (control group), contaminated groups (nonsterilization group [group 1], ethylene oxide [EO] gas group [group 2], and autoclave group [group 3]). The bone-to-implant contact (BIC) and the new bone formation rate (NBFR) were evaluated after sacrifice.
Results:
The BIC and NBFR of groups 1 and 3 were significantly lower than the control group after 4 weeks. The BIC and NBRF of group 3 were significantly lower than the control group after 8 weeks. However, the BIC and NBRF of group 2 was not significantly different comparing with the control group after 4 and 8 weeks.
Conclusion:
Sterilization using EO gas may be more favorable than high-pressure sterilization in cases the reuse of contaminated autogenous tooth graft materials.
