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Updated: Mar 30, 2026
Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
Published on: July 1, 2011
[Evaluation of a reduced panel of leptospira strains for microagglutination]
Paulina Jacob1, María F Schmeling1, Yosena T Chiani1
1Laboratorio de Leptospirosis, Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni, Santa Fe, Argentina.
Objective:
To evaluate if the use of the 19 Leptospira strains panel suggested by the International Leptospirosis Society of World Health Organization for microagglutination allows confirmation of more cases that the 12 strains panel used in Argentina.
Materials And Methods:
Cross-sectional observational study. We studied 441 serum samples corresponding to Argentinean patients with suspected leptospirosis derived during from July to December, 2009 and from January to October, 2013.
Results:
The same number of positive samples was obtained using the MAT with the 19 or 12 strains. In six cases a serovar of the expanded collection was presumably infecting, but always coagglutinated with strains of the reduced panel.
Conclusion:
In Argentina, the diagnosis of leptospirosis by MAT could be made using the reduced 12 strains panel, obtaining the same result in case detection as using the 19 strains panel. Additional information provided by the use of all strains could be the presumably infecting serogroup.
