Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

8.5K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
8.5K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

19.5K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
19.5K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

4.3K
4.3K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

11.4K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
11.4K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

4.2K
4.2K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

25.2K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
25.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comprehensive metabolomics and flavoromics analysis reveal the changes in muscle flavor quality of turbot (Scophthalmus maximus) during low-temperature waterless live transport.

Food research international (Ottawa, Ont.)·2026
Same author

Development and Evaluation of a Promising Biomarker for Diagnosis of Latent and Active Tuberculosis Infection.

Infectious diseases & immunity·2026
Same author

Multi-omics and machine learning-based exploration of key genes associated with abdominal aortic aneurysm.

Frontiers in molecular biosciences·2026
Same author

Successful treatment of refractory focal eosinophilic myositis with extra-ocular muscle involvement using tocilizumab: a case report.

Frontiers in immunology·2026
Same author

Combined Oat β-Glucan and Soy Protein Isolate Reprogram Gut Microbiota and Improve Metabolic Dysfunction in Diet-Induced Obesity.

Nutrients·2026
Same author

Plasma SERPINA3 and its clinical correlates in adolescent major depressive disorder: an exploratory case-control study.

BMC psychiatry·2026

Related Experiment Video

Updated: Mar 30, 2026

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

33.7K

Fragile Nucleosomes Influence Pol II Promoter Function.

Suman K Pradhan1, Yong Xue1, Michael F Carey1

  • 1Department of Biological Chemistry, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.

Molecular Cell
|November 7, 2015
PubMed
Summary

The RSC chromatin remodeling complex and DNA motifs create fragile nucleosomes at yeast Pol II promoters. This mechanism differs from promoters with stable nucleosomes, offering new insights into gene regulation.

More Related Videos

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

10.3K
Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

3.2K

Related Experiment Videos

Last Updated: Mar 30, 2026

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

33.7K
Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

10.3K
Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

3.2K

Area of Science:

  • Molecular biology
  • Chromatin dynamics
  • Gene regulation

Background:

  • Nucleosomes play a crucial role in regulating gene accessibility.
  • Chromatin remodeling complexes are essential for dynamic changes in chromatin structure.
  • Understanding promoter nucleosome stability is key to deciphering transcription regulation.

Purpose of the Study:

  • To investigate the mechanism by which the RSC chromatin remodeling complex influences nucleosome assembly at yeast Pol II promoters.
  • To differentiate between fragile and stable nucleosome formation at different promoter types.
  • To identify the roles of DNA sequence motifs and regulatory factors in this process.

Main Methods:

  • Chromatin immunoprecipitation (ChIP) assays.
  • Nucleosome mapping techniques.
  • Analysis of DNA sequence motifs and transcription factor binding.

Main Results:

  • The RSC complex, in conjunction with specific DNA motifs and general regulatory factors, facilitates the assembly of fragile nucleosomes.
  • Fragile nucleosomes are characteristic of highly transcribed yeast Pol II promoters.
  • Promoters with stable nucleosomes exhibit distinct regulatory features.

Conclusions:

  • The RSC complex is a key player in establishing nucleosome lability at active yeast promoters.
  • Specific DNA sequences and regulatory factors cooperate with RSC to control nucleosome stability.
  • This study provides a framework for distinguishing promoter types based on nucleosome dynamics.