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Molecular cloning and expression of the O4 polysaccharide gene cluster from Escherichia coli
G E Haraguchi1, R A Hull, U Krallmann-Wenzel
1Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030.
Microbial Pathogenesis
|February 1, 1989
Abstract:
The Escherichia coli O4 serotype is common among isolates from urinary tract infections. The genes responsible for the biosynthesis of the O4 polysaccharide in a human uropathogenic E. coli were cloned and expressed in E. coli K-12. The recombinant plasmid pGH60, which conferred the O4 phenotype, encoded eight proteins with apparent molecular weights of 39, 36.5, 35, 32.8, 26, 24, 20.7 and 13 kDa.