Evaluation of kudzu root extract-induced hepatotoxicity
Dengyuan Wang1, Liang Qiu2, Xiaoli Wu2
1State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Journal of Ethnopharmacology
|November 8, 2015
Summary
Kudzu root extract may cause liver damage. Studies in mice and liver cells showed increased liver enzymes and cell damage, indicating potential hepatotoxicity.
Area of Science:
- Toxicology
- Pharmacology
- Hepatology
Background:
- Kudzu root (Pueraria lobata) has a long history of use in traditional medicine.
- Limited research suggests potential liver damage associated with kudzu root consumption.
Purpose of the Study:
- To investigate the hepatotoxicity of kudzu root extract.
- To evaluate the effects of kudzu root extract on liver cells in vitro and in vivo.
Main Methods:
- Mice were administered kudzu root extract (10mg/day) for 4 weeks, followed by biochemical and histopathological analysis.
- HepG2 cells and primary mouse hepatocytes were co-cultured with kudzu root extract or puerarin to assess cellular toxicity and mechanisms.
Main Results:
- Hepatotoxicity was confirmed by elevated serum ALT and AST levels and observed histopathological changes in mice.
- Increased lactate dehydrogenase (LDH) leakage in HepG2 cells and hepatocytes further supported the hepatotoxic effects of kudzu root extract.
- Upregulation of Mt1 mRNA and Bax mRNA was observed, suggesting involvement in acute phase response and apoptosis.
Conclusions:
- Kudzu root extract demonstrates hepatotoxic potential.
- Caution is advised when using kudzu root, due to its possible adverse effects on the liver.
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